Abstract
This study examined the antiangiogenic capacity of diosmin in vitro and their interactions with certain important receptors related to cancer and infectious diseases via molecular docking. Diosmin, a naturally occurring flavone glycoside, largely found in citrus fruits, exhibits potentially antiangiogenic properties especially interacting with both VEGF and VEGFR. Treatment with certain doses of diosmin caused a reduction with a ratio of 22–68% on VEGF levels as compared to non-treated control groups. Western blotting and real-time PCR results demonstrated that protein expression of VEGF was downregulated in a dose- and time-dependent manner. On the other hand, we aimed to check the possible interaction of diosmin with VEGF, VEGFR, HER, PR, ER, and Omp-a, receptor proteins. According to our molecular docking results, diosmin effectively binds these receptors and probably this could be one of the possible reasons for its anti-angiogenic and anti-microbial properties.
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All the authors have read and approved the final submission. EŞ: hypothesis, conceptualization, ELISA, Western blotting, real-time PCR, molecular docking, writing, review, and editing; OK: literature searching, cell culture, ELISA, western blotting, molecular docking, statistic, original draft; KY: ELISA, real-time PCR; AK: cell culture, ELISA, western blotting; NDT: molecular docking, literature searching, original draft; SB: literature searching, cell culture, ELISA; NI: real-time PCR; CA: statistic, writing; ETA: western blotting, real-time PCR; MÇ: statistic, graphs, editing; AYÇ: conceptualization, review, editing.
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Şimşek, E., Koçak, O., Yıldırım, K. et al. The Anti-angiogenic and Anti-microbial Effect of Diosmin: Potential Receptor Interactions via Molecular Docking. Rev. Bras. Farmacogn. 33, 422–431 (2023). https://doi.org/10.1007/s43450-023-00365-y
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DOI: https://doi.org/10.1007/s43450-023-00365-y