Abstract
Myocardial infarction is a type of cardiovascular disease that leads to the loss of cardiomyocyte and cardiac dysfunction and is classified as the leading cause of death. This study investigated the effect of capillarisin, a bioactive ingredient of Artemisia capillaris Thunb., Asteraceae, root extracts, on myocardial infarction development in a rat model. Capillarisin treatment lessened the myocardial infarct size and improved cardiac function in myocardial infarction rats by lowering creatine kinase-MB, lactate dehydrogenase, and malondialdehyde levels, and elevating the level of superoxide dismutase in serum, suggesting its inhibitory effect on oxidative stress. Capillarisin also inhibited inflammation and cardiomyocyte apoptosis in myocardial tissues. The decreased protein levels of phosphatidylinositol 3-kinase and phosphorylation of protein kinase B in myocardial infarction rats were upregulated by capillarisin treatment. Capillarisin reduced the cardiomyocyte apoptosis and mitigated oxidative stress and inflammation in myocardial infarction rats by activating phosphatidylinositol 3-kinase and phosphorylation of protein kinase B signaling pathway.
Graphical Abstract
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahn JH, Hyun JY, Jeong MH, Kim JH, Hong YJ, Sim DS, Kim MC, Park HS, Kim DI, Hur SH, Oh SK, Ahn Y (2021) Comparative effect of angiotensin converting enzyme inhibitor versus angiotensin ii type i receptor blocker in acute myocardial infarction with non-obstructive coronary arteries; from the Korea Acute Myocardial Infarction Registry-National Institute of Health. Cardiol J 28:738–745. https://doi.org/10.5603/CJ.a2020.0130
Bao YR, Jiang WY, Yu JY, Chen JW, Zhang GX (2020) Traditional Chinese medicine formulation Huangqi Jianzhong Tang improves cardiac function after myocardial infarction in rats. Evid Based Complement Alternat Med 2020:3106076. https://doi.org/10.1155/2020/3106076
Christia P, Frangogiannis NG (2013) Targeting inflammatory pathways in myocardial infarction. Eur J Clin Invest 43:986–995. https://doi.org/10.1111/eci.12118
Han S, Lee JH, Kim C, Nam D, Chung WS, Lee SG, Ahn KS, Cho SK, Cho M, Ahn KS (2013) Capillarisin inhibits iNOS, COX-2 expression, and proinflammatory cytokines in LPS-induced RAW 264.7 macrophages via the suppression of ERK, JNK, and NF-κB activation. Immunopharmacol Immunotoxicol 35:34–42. https://doi.org/10.3109/08923973.2012.736522
Hao P, Jiang F, Cheng J, Ma L, Zhang Y, Zhao Y (2017) Traditional Chinese medicine for cardiovascular disease: evidence and potential mechanisms. J Am Coll Cardiol 69:2952–2966. https://doi.org/10.1016/j.jacc.2017.04.041
Hashmi S, Al-Salam S (2015) Acute myocardial infarction and myocardial ischemia-reperfusion injury: a comparison. Int J Clin Exp Pathol 8:8786–8796
Hausenloy DJ, Barrabes JA, Bøtker HE, Davidson SM, Di Lisa F, Downey J, Engstrom T, Ferdinandy P, Carbrera-Fuentes HA, Heusch G, Ibanez B, Iliodromitis EK, Inserte J, Jennings R, Kalia N, Kharbanda R, Lecour S, Marber M, Miura T et al (2016) Ischaemic conditioning and targeting reperfusion injury: a 30 year voyage of discovery. Basic Res Cardiol 111:70. https://doi.org/10.1007/s00395-016-0588-8
He F, Zuo L (2015) Redox roles of reactive oxygen species in cardiovascular diseases. Int J Mol Sci 16:27770–27780. https://doi.org/10.3390/ijms161126059
Hori M, Nishida K (2009) Oxidative stress and left ventricular remodelling after myocardial infarction. Cardiovasc Res 81:457–464. https://doi.org/10.1093/cvr/cvn335
Hwa JS, ** YC, Lee YS, Ko YS, Kim YM, Shi LY, Kim HJ, Lee JH, Ngoc TM, Bae KH, Kim YS, Chang KC (2012) 2-Methoxycinnamaldehyde from Cinnamomum cassia reduces rat myocardial ischemia and reperfusion injury in vivo due to HO-1 induction. J Ethnopharmacol 139:605–615. https://doi.org/10.1016/j.jep.2011.12.001
Khan S, Choi RJ, Shehzad O, Kim HP, Islam MN, Choi JS, Kim YS (2013) Molecular mechanism of capillarisin-mediated inhibition of MyD88/TIRAP inflammatory signaling in in vitro and in vivo experimental models. J Ethnopharmacol 145:626–637. https://doi.org/10.1016/j.jep.2012.12.001
Kim J, Lim J, Kang BY, Jung K, Choi HJ (2017) Capillarisin augments anti-oxidative and anti-inflammatory responses by activating Nrf2/HO-1 signaling. Neurochem Int 105:11–20. https://doi.org/10.1016/j.neuint.2017.01.018
Komiya T, Tsukui M, Oshio H (1975) Letter: capillarisin, a constituent from Artemisiae capillaris herba. Chem Pharm Bull 23:1387–1389. https://doi.org/10.1248/cpb.23.1387
Lai XX, Zhang N, Chen LY, Luo YY, Shou BY, **e XX, Liu RH (2020) Latifolin protects against myocardial infarction by alleviating myocardial inflammatory via the HIF-1α/NF-κB/IL-6 pathway. Pharm Biol 58:1156–1166. https://doi.org/10.1080/13880209.2020.1840597
Lee TY, Chen FY, Chang HH, Lin HC (2009) The effect of capillarisin on glycochenodeoxycholic acid-induced apoptosis and heme oxygenase-1 in rat primary hepatocytes. Mol Cell Biochem 325:53–59. https://doi.org/10.1007/s11010-008-0019-8
Lefer DJ, Granger DN (2000) Oxidative stress and cardiac disease. Am J Med 109:315–323. https://doi.org/10.1016/s0002-9343(00)00467-8
McIlwain DR, Berger T, Mak TW (2013) Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol 5:a008656. https://doi.org/10.1101/cshperspect.a008656
Meng H, Zhang Y, An ST, Chen Y (2019) Annexin A3 gene silencing promotes myocardial cell repair through activation of the PI3K/Akt signaling pathway in rats with acute myocardial infarction. J Cell Physiol 234:10535–10546. https://doi.org/10.1002/jcp.27717
Nagaoka K, Matoba T, Mao Y, Nakano Y, Ikeda G, Egusa S, Tokutome M, Nagahama R, Nakano K, Sunagawa K, Egashira K (2015) A new therapeutic modality for acute myocardial infarction: nanoparticle-mediated delivery of pitavastatin induces cardioprotection from ischemia-reperfusion injury via activation of PI3K/Akt pathway and anti-inflammation in a rat model. PLoS ONE 10:e0132451. https://doi.org/10.1371/journal.pone.0132451
Reed GW, Rossi JE, Cannon CP (2017) Acute myocardial infarction. Lancet 389:197–210. https://doi.org/10.1016/s0140-6736(16)30677-8
Ren BC, Zhang YF, Liu SS, Cheng XJ, Yang X, Cui XG, Zhao XR, Zhao H, Hao MF, Li MD, Tie YY, Qu L, Li XY (2020) Curcumin alleviates oxidative stress and inhibits apoptosis in diabetic cardiomyopathy via Sirt1-Foxo1 and PI3K-Akt signalling pathways. J Cell Mol Med 24:12355–12367. https://doi.org/10.1111/jcmm.15725
Rodrigo R, Libuy M, Feliú F, Hasson D (2013) Molecular basis of cardioprotective effect of antioxidant vitamins in myocardial infarction. Biomed Res Int 2013:437613. https://doi.org/10.1155/2013/437613
Roth GA, Mensah GA, Fuster V (2020) The global burden of cardiovascular diseases and risks: a compass for global action. J Am Coll Cardiol 76:2980–2981. https://doi.org/10.1016/j.jacc.2020.11.021
Sabatine MS, Braunwald E (2021) Thrombolysis in myocardial infarction (TIMI) study group: JACC Focus Seminar 2/8. J Am Coll Cardiol 77:2822–2845. https://doi.org/10.1016/j.jacc.2021.01.060
Sabatine MS, Bergmark BA, Murphy SA, O’Gara PT, Smith PK, Serruys PW, Kappetein AP, Park SJ, Park DW, Christiansen EH, Holm NR, Nielsen PH, Stone GW, Sabik JF, Braunwald E (2021) Percutaneous coronary intervention with drug-eluting stents versus coronary artery bypass grafting in left main coronary artery disease: an individual patient data meta-analysis. Lancet 398:2247–2257. https://doi.org/10.1016/s0140-6736(21)02334-5
Surekha RH, Srikanth BB, Jharna P, Ramachandra RV, Dayasagar RV, Jyothy A (2007) Oxidative stress and total antioxidant status in myocardial infarction. Singapore Med J 48:137–142
Xu S, Wu B, Zhong B, Lin L, Ding Y, ** X, Huang Z, Lin M, Wu H, Xu D (2021) Naringenin alleviates myocardial ischemia/reperfusion injury by regulating the nuclear factor-erythroid factor 2-related factor 2 (Nrf2)/System xc-/glutathione peroxidase 4 (GPX4) axis to inhibit ferroptosis. Bioengineered 12:10924–10934. https://doi.org/10.1080/21655979.2021.1995994
Yamahara J, Kobayashi G, Matsuda H, Katayama T, Fujimura H (1989) Vascular dilatory action of Artemisia capillaris bud extracts and their active constituent. J Ethnopharmacol 26:129–136. https://doi.org/10.1016/0378-8741(89)90060-3
Yang Y, Li J, Rao T, Fang Z, Zhang J (2021) The role and mechanism of hyperoside against myocardial infarction in mice by regulating autophagy via NLRP1 inflammation pathway. J Ethnopharmacol 276:114187. https://doi.org/10.1016/j.jep.2021.114187
Yu Z, Tang L, Chen L, Li J, Wu W, Hu C (2015) Capillarisin suppresses lipopolysaccharide-induced inflammatory mediators in BV2 microglial cells by suppressing TLR4-mediated NF-κB and MAPKs signaling pathway. Neurochem Res 40:1095–1101. https://doi.org/10.1007/s11064-015-1567-4
Zhang P, Liu X, Huang G, Bai C, Zhang Z, Li H (2017) Barbaloin pretreatment attenuates myocardial ischemia-reperfusion injury via activation of AMPK. Biochem Biophys Res Commun 490:1215–1220. https://doi.org/10.1016/j.bbrc.2017.06.188
Zhao T, Wang Q (2020) Capillarisin protects SH-SY5Y cells against bupivacaine-induced apoptosis via ROS-mediated PI3K/PKB pathway. Life Sci 259:118279. https://doi.org/10.1016/j.lfs.2020.118279
Author information
Authors and Affiliations
Contributions
BR, SQ, and YW designed the study. BR and SQ performed the research method and analyzed the data. YW wrote the paper. BR and SQ were responsible for polishing the manuscript. All the authors have discussed the results and approved the final manuscript.
Corresponding author
Ethics declarations
Ethics Approval
This experiment was reviewed by the Animal Ethics Committee of Hubei Provincial Center for Disease Control and Prevention (approval number: 202220012). All experimental were conducted in accordance with the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Ruan, B., Qiu, S. & Wang, Y. Capillarisin Pretreatment Inhibits Oxidative Stress and Inflammation in Rats After Myocardial Infarction Induction. Rev. Bras. Farmacogn. 32, 984–992 (2022). https://doi.org/10.1007/s43450-022-00306-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s43450-022-00306-1