Abstract
Lysiphyllum strychnifolium (W. G. Craib) A. Schmitz (syn. Bauhinia strychnifolia), Fabaceae, vernacularly known in Thai as “Ya-Nang-Daeng,” has been traditionally used for detoxification and longevity. A high-performance liquid chromatography for the determination of four bioactive components, gallic acid, trilobatin, yanangdaengin, and astilbin, as chemical markers for leaves and stems of this medicinal plant, was developed and validated. The chromatographic separation was performed on a reversed-phase C-18 column using a gradient system of 0.5% acetic acid and methanol with a flow rate of 1 ml/min. The validated method showed good sensitivity, linearity, precision, and accuracy. The effect of solvent polarity on the yield of bioactive compounds was also studied. The gallic acid, trilobatin, and yanangdaengin contents of L. strychnifolium leaves as well as astilbin from its stems collected from different localities were analyzed. Chromatographic fingerprinting between each population indicated a homogeneity of L. strychnifolium in Thailand.
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Acknowledgements
The authors greatly acknowledge the financial support provided by the Thammasat University Research Fund under the TU Research Scholar, Contract No. 2/29/2561, and Thammasat University Research Unit in Cannabis and Herbal Products Innovation. We thank Ms. Pajaree Inthachub for plant collection, discussion, and identification. We thank the Drug Discovery and Development Center, Office of Advanced Science and Technology, for the laboratory facilities.
Funding
Thammasat University Research Fund under the TU Research Scholar, Contract No. 2/29/2561, and Thammasat University Research Unit in Cannabis and Herbal Products Innovation.
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SK, NT, WI, ND, and SC collected the samples and performed the laboratory work. SK, NT, and ND designed and analyzed the results. SK, ND, SC, and VK discussed the results. SK, ND, and SC wrote the manuscript. All of the authors read and approved the final submission.
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Kongkiatpaiboon, S., Tayana, N., Inthakusol, W. et al. HPLC Quantification of Chemical Markers from Lysiphyllum strychnifolium. Rev. Bras. Farmacogn. 32, 266–272 (2022). https://doi.org/10.1007/s43450-022-00237-x
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DOI: https://doi.org/10.1007/s43450-022-00237-x