Abstract
Copaifera species display several biological activities, such as antimicrobial, antinociceptive, gastroprotective, and anti-inflammatory. Some of these activities are directly associated with the presence of compounds with significant antioxidant properties. Determination of the chemical composition of the leaf extracts of nine Copaifera species was performed, by attributing which compounds are responsible for the antioxidant activity based on metabolomic analysis, through qualitative chemical analysis by liquid chromatography coupled with high-resolution mass spectrometry and in vitro antioxidant activity assay. Eighty-one compounds were found in all analyzed extracts, being 60 peaks totally identified as phenolic compounds (galloylquinic acids and flavonoids), organic acids, coumarins, and terpenes. Orthogonal partial least-squares discriminant analysis indicated that six galloylquinic acids and one flavonoid were the most active compounds. The chemical composition of the nine Copaifera species leaves revealed different classes of compounds.
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Acknowledgements
We thank Milton Groppo (FFCLRP-USP, Brazil), Haroldo C. de Lima (SPFR Herbarium), Regina C. V. Martins-da-Silva, and Silvane T. Rodrigues (EMBRAPA Amazônia Oriental) for the identification of plants, and Dr. Fernando Batista da Costa (FCFRP-USP, Brazil) for the support in the LC-MS/MS analyzes. We also acknowledge the essential technical support of José Roberto Jabor, Luiz F. da Silva, Maria Angélica S. C. Chellegatti, Mário S. Ogasawara, and Walter Lopes (FCFRP technical team). Authorizations for the collection and research of plant material were requested and granted by the Brazilian Council for Authorization and Information on Biodiversity (SIBIO/ICMBio/MMA/BRASIL) and Genetic Heritage Management (CGEN/MMA/BRASIL) through documents 35143-1 and 010225/2014-5, respectively.
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Financial support and scholarships were granted by the São Paulo Research Foundation (FAPESP) (Process number 2011/13630-7) and Coordination for the Improvement of Higher Education Personnel (CAPES) for both.
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JJMS was responsible for collecting the plants, idealizing and develo** experiments, treating results, and writing the first version of the manuscript. RC guided and carried out chemometrics treatment of the LC–MS/MS experiments. SAF and MJVF supported in the antioxidant analyzes. HR and VCGH helped in the treatment and discussion of results. JKB supervised and guided the study. All authors reviewed the final version of the manuscript and approved the submission.
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This article is part of a Special Issue to celebrate the 35th anniversary of the Brazilian Journal of Pharmacognosy.
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da Silva, J.J.M., Casoti, R., Figueiredo, S.A. et al. Determination of the Composition of Copaifera (Fabaceae) Leaf Extracts with Potential Antioxidant Activity by Metabolomics Approach. Rev. Bras. Farmacogn. 31, 720–725 (2021). https://doi.org/10.1007/s43450-021-00201-1
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DOI: https://doi.org/10.1007/s43450-021-00201-1