Abstract
In this work, we studied the potential photoprotective effect of Ipomoea horsfalliae Hook., Convolvulaceae, flower extract. Ipomoea horsfalliae is a plant that grows in tropical and subtropical regions. I. horsfalliae ethanolic extracts were analyzed by ultra-high efficiency liquid chromatography–high-resolution mass spectrometry. Dicaffeoylquinic acid, chlorogenic acid, scopoletin, glycosylated cyanidin, pelargonidin, and kaempferol were identified as major components of I. horsfalliae flower extract. In vitro biossays were used to evaluate cytotoxic and sensitizing effects of the extracts, and their photoprotective effect was evaluated in BALB/c mice. Morphological and histopathological observation of the skin tissues from mice suggested that UV-B-induced edema was significantly inhibited by treatment with I. horsfalliae flower extract. It was not cytotoxic for both cancerous and normal cells, and no sensitizing effect was observed. I.horsfalliae flower extract appears to be a good starting point for research programs leading to the development of natural skin care products.
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Funding
This work was supported by COLCIENCIAS [Convocations 617 and 647 Doctorados Nacionales]; Ministerio de Educación Nacional, Ministerio de Industria, Comercio y Turismo, ICETEX, Convocatoria Ecosistema Científico-Colombia Científica; COLCIENCIAS [Grant RC-FP44842-212-2018].
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LJS, YC, and JJM contributed to running the laboratory work and chromatographic and data analysis. LJS drafted the manuscript. EQ and REO contributed to evaluating cytotoxic and sensitizing activities. REO supervised the laboratory work and aided in the drafting of the paper. JGA, AMG, and AME contributed to evaluating photoprotective activity and critical reading of the manuscript. JCB and MRG contributed to histological analysis. JRM and EES contributed to the analytical study, supervised the laboratory work, and carried out critical reading and manuscript drafting.
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Plant material was collected according to a Contract for Access to Genetic Resources and Derivative Products (No. 101, June 3, 2014, Colombian Ministry of Environment and Sustainable Development).
Protection of Human and Animal Subjects
The photoprotective test was endorsed by the Ethics Committee (September 5, 2017) and the Biosafety Commission (September 13, 2017) of the National Autonomous University of Mexico. All procedures were carried out in accordance with Official Mexican Regulation (NOM-062-ZOO-1999).
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Sierra, L.J., Córdoba, Y., Mejía, J.J. et al. Photoprotective Activity of Ipomoea horsfalliae Flower Extract. Rev. Bras. Farmacogn. 30, 69–79 (2020). https://doi.org/10.1007/s43450-020-00024-6
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DOI: https://doi.org/10.1007/s43450-020-00024-6