Abstract
Caseous lymphadenitis is a well-known disease caused by Corynebacterium pseudotuberculosis affecting small ruminants with small significance to human health because of its minor zoonotic potential. In both cases, few treatment options are available and conventional antimicrobial therapy is commonly refractory due to development of pyogranulomatous reactions, bringing great interest in discovering novel therapeutics for more suitable approaches. Dideoxynucleotides presented antibacterial action against various bacteria but were never described for C. pseudotuberculosis. Hypothesizing the antimicrobial action of 2’,3’-dideoxiadenosine (ddATP) against C. pseudotuberculosis, we performed for the first time an investigation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ATCC® 19,410 strain and a well-characterized clinical isolate of C. pseudotuberculosis. We also assessed potential synergism with penicillin. ddATP showed a growth delay effect for C. pseudotuberculosis at 2 µmol/mL and a MIC and MBC of 4 µmol/mL against the ATCC® 19,410 strain, but not for the clinical strain. An antimicrobial effect was observed when using concentrations lower than the MIC of ddATP associated with penicillin for both strains tested. Our data suggest the potential of nucleotide analogs, especially adenosine, and its combination with penicillin, as a possible novel treatment for C. pseudotuberculosis-induced infections, and contributes with knowledge regarding alternative drugs to treat C. pseudotuberculosis infections.
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The National Council for Scientific and Technological Development (CNPq) provided a scholarship for PNB (132282/2019). This funding source had no involvement in study design, development, or decisions.
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PNB and JPOF contributed to the conception and design of the study. PNB and CLP contributed to the samples collection. PNB, CLP, MGR, VACA, and AFJ contributed to the processing of the samples. PNB, ASB, and JPOF contributed to writing the first drafts of the manuscript. All authors contributed to manuscript revision, and read and approved the submitted version.
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Bernardino, P.N., de Paula, C.L., Pereira, A.F.M. et al. Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis. Braz J Microbiol 54, 559–563 (2023). https://doi.org/10.1007/s42770-022-00885-0
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DOI: https://doi.org/10.1007/s42770-022-00885-0