Abstract
Molybdenum (Mo) deficiency in the farmland can limit biological nitrogen fixation (BNF) and at the same time restricts the availability and uptake of nitrogen (N) by roots. However, the impact of Mo application on N bioavailability or BNF capacity in wheat-soil systems is still unclear. Mo was applied to the soil as 0 (− Mo) and 0.15 mg kg−1 soil (+ Mo) to investigate the impacts of Mo application on δ15N natural abundance in the wheat-soil system, and the activities of soil enzymes such as nitrogenase, nitrate reductase, and microbial biomass of N, and photosynthetic variables. Mo application increased the net photosynthetic rate, intercellular CO2 concentration, nitrogenase and nitrate reductase activities, and microbial biomass of N in wheat-soil system, suggesting that Mo enhances N bioavailability and reduces nitrate (NO3−) accumulation as well as improving the efficiency of N metabolism in wheat. Hence, N uptake and N concentrations were significantly increased among wheat tissues under Mo supply, except for root N concentration. Mo application significantly increased 15N fixation and δ15N abundance in soil by 92.2% and 10.8%, while in plant it is increased by 381.6% and 13.9%, respectively as compared with − Mo treatment, indicating that Mo application is vital for nitrogenase enzyme activity which converts atmospheric N2 into bioavailable NH3 and to provide N to terrestrial ecosystems. Mo plays a vital role in increasing BNF in Mo-deficient soil (≤ 0.082 mg kg−1) and Mo-insufficient wheat. These findings suggest that Mo application may increase soil N input in wheat-soil systems under Mo deficiency conditions.
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Acknowledgements
The authors thank Prof. Soliman M. Soliman., Prof. Yehia G. M. Galal., and Prof. Hussein A. Abdelaziz., from Soil & Water Research Department, Egyptian Atomic Energy Authority, for valuable suggestions to improve the early version of the manuscript. The authors would also like to thank Dr. Ibrahim A. A. Mohamed, Faculty of Agriculture, Fayoum University, for his suggestions that helped us to improve the manuscript.
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This work was supported by Hubei Province Technological Innovation Major Project (2018ABA079), the National Natural Science Foundation of China (Program No. 42077095), and Open Project for Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization (202020404), MGM, acknowledge CSC for his PhD scholarship (CSC No 2017GBJ001669).
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M. G. M., C. H., and X. S. conceived and designed the experiment; M. G. M., S. E., and A. M. conducted the experiment; M. H. S., M. R., Z. D., L. H., and M. A. I. handled data curation, analysis, and visualization; S. E. and X. S. provided statistical guidance; M. G. M. and M. A. I. wrote the manuscript; all authors have revised the manuscript.
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Moussa, M.G., Sun, X., El-Tohory, S. et al. Molybdenum Role in Nitrogen Bioavailability of Wheat-Soil System Using the Natural 15N Abundance Technique. J Soil Sci Plant Nutr 22, 3611–3624 (2022). https://doi.org/10.1007/s42729-022-00913-w
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DOI: https://doi.org/10.1007/s42729-022-00913-w