Abstract
Maturity of the seeds can affect their potential longevity. It has been shown that components related to seed quality develop after the moment when the seed has reached its maximum dry mass, known as harvest maturity. In this state seeds have already developed critical adaptation mechanisms, such as detoxification of reactive oxygen species (ROS), which allow it to survive accelerated water loss. The objective of the present study was to determine the effect of maturity on physiological and biochemical indicators associated with the germination and vigour of tobacco seed. Tobacco seeds were collected at 14, 21, 28, 35, 42, 49 and 56 days after anthesis, the moisture content, dry and fresh mass of 1000 seeds and germination power were determined and the vigour of the seeds was evaluated through accelerated aging test and electrolytes leakage. In addition, generation rate of O2·− and H2O2 content were determined, as well as enzyme activity Superoxide Dismutase and Catalase. Tobacco seeds collected at 35 DAA showed superior vigor as a result of greater tolerance to accelerated aging conditions and cell membrane integrity, reflected in decreased electrolyte leakage values, as a consequence of an adequate oxidative balance. The results showed that there is a close relationship between antioxidant enzyme activity, especially CAT activity, and seed development. CAT enzyme activity levels suggest that hydrogen peroxide scavenging may be crucial to avoid oxidative stress on the way to the acquisition of desiccation tolerance in tobacco seeds.
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JLPR: conducted the study, participated in the search for funding to support the project and contributed to the experimental design, acquisition, analysis and interpretation of data (statistical assessment). He wrote the first draft of the manuscript and contributed providing detailed comments, additions, and text modifications as the manuscript developed. RGR and GYL: contributed to the planning, design, analysis, and interpretation of the data. JLG: was involved in the design of the experiments and data management to align with the original concept of the work and participated in the writing of manuscript. MMM: was involved in sourcing funding to support the project and in the conception and design of the experiments. He also participated in obtaining and interpreting the data and contributed detailed comments, additions and modifications to the text as the manuscript was developed. All authors read and approved the final manuscript.
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Pérez-Rodríguez, J.L., Ramos Aquino, R.G., Lorente González, G.Y. et al. ROS production and antioxidant enzyme activity in relation to germination and vigor during tobacco seed development. Vegetos 36, 506–515 (2023). https://doi.org/10.1007/s42535-022-00412-4
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DOI: https://doi.org/10.1007/s42535-022-00412-4