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Occurrence and Ecotoxicological Impacts of Fluoroquinolone Antibiotics in Leachates from Three Nigerian Dumpsites

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Abstract

Solid waste dumpsites receive wastes containing pharmaceuticals and therefore serve as reservoirs of antibiotic residues. Leachates produced from dumpsites are among the principal means of transferring antibiotic residues into the aquatic and soil ecosystems. The occurrence, contamination level and ecotoxicological risk of three fluoroquinolone antibiotics (ciprofloxacin, norfloxacin and ofloxacin) in leachates from three dumpsites in Ibadan, South-Western Nigeria were investigated. An optimized quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction was adopted for the extraction of target fluoroquinolones, extract was cleaned-up by dispersive-solid phase extraction while instrumental analysis was achieved using high performance liquid chromatography (HPLC). Risk quotient approach was used to estimate the potential ecotoxicological risk of target fluoroquinolone antibiotics to aquatic organisms at three trophic levels (algae, daphnid and fish). Recoveries ranged from 102–128%. Method limits of quantification were 0.35, 1.91 and 11.69 μg L−1 for norfloxacin, ciprofloxacin and ofloxacin, respectively. Inter-day precision (% RSD) ranged from 7–22%. High levels of studied fluoroquinolones were quantified, up to 796 μg L−1 for ofloxacin in leachates from Aba-Eku dumpsite. Based on chronic (long-term) toxicity data, norfloxacin in the leachates posed high risk to the three aquatic organisms. Ciprofloxacin presented moderate risk to algae in only Aba-Eku leachate in spite of the low risk observed for daphnids and fish in leachates from the three investigated dumpsites. Considering the high concentrations and potential risk of target fluoroquinolones in the leachates, groundwater resources around the investigated dumpsites should be monitored in future studies for these antibiotics and other pharmaceuticals.

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Data availability

Data will be made available on request.

Abbreviations

QuEChERS:

Quick, easy, cheap, effective, rugged and safe

HPLC:

High performance liquid chromatography

LOQ:

Limit of quantification

LOD:

Limit of detection

RSD:

Relative standard deviation

ARB:

Antibiotic resistant bacteria

ARGs:

Antibiotic resistance genes

SPE:

Solid phase extraction

dSPE:

Dispersive solid phase extraction

RQ:

Risk quotient

MEC:

Measured environmental concentrations

PNEC:

Predicted no effect concentrations

NOEC:

No observed effect concentration

PSA:

Primary and secondary amine

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Acknowledgements

We acknowledge the assistance rendered by the staff of the investigated dumpsites in samples collection.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial or not-for-profit sectors.

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Authors and Affiliations

  1. Analytical/Environmental Chemistry Unit, Department of Chemistry, University of Ibadan, Ibadan, Nigeria

    Akinranti S. Ajibola, Juliana B. Agberotimi & Aanuoluwapo I. Obanubi

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  1. Akinranti S. Ajibola
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Correspondence to Akinranti S. Ajibola.

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Ajibola, A.S., Agberotimi, J.B. & Obanubi, A.I. Occurrence and Ecotoxicological Impacts of Fluoroquinolone Antibiotics in Leachates from Three Nigerian Dumpsites. Chemistry Africa 7, 443–453 (2024). https://doi.org/10.1007/s42250-023-00748-7

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