Abstract
Black shank disease incited by Phytophthora nicotianae is one of the most devastating soil-borne diseases of tobacco (Nicotiana tabacum L.). Crop rotation is an effective strategy for the management of this disease. However, the efficacy of rotation with different crops in controlling tobacco black shank remains obscure. In this study, 10 different preceding crops, including rape, wheat, barley, broad bean, pea, garlic, ryegrass, cow vetch, alfalfa, and milk vetch, were selected in fields with an history of severe tobacco black shank. The effectiveness of crop rotation on tobacco black shank disease control was quantitatively determined as amount of Phytophthora nicotianae using (1) fluorescence quantitative Polymerase Chain Reaction (qPCR), (2) tobacco leaf disk assays, and (3) soil dilution plating assays. In addition, the bacterial assemblage associated with rhizosphere of different crops was analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). Results showed that ryegrass, garlic, and barley can effectively reduce the levels of P. nicotianae in the soil, with a consequent decrease of both morbidity and disease index. The rhizosphere bacterial community assemblages changed depending on the different preceding crops.
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This research was funded by the Yunnan Academy of Tobacco Agricultural Sciences (grant numbers 2020530000241012, 2019530000241007, 2020530000241013 and 2022530000241019), Yunnan Provincial Science and Technology Department (grant numbers 202001AU070012) and National Natural Science Foundation of China (grant numbers 32102161).
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D.F. prepared the materials and conducted the experiments; data analyzed by Z.X; X.G. wrote the original draft with C.H and C.L. review and editing. All authors read and approved the final manuscript.
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Gai, XT., Lu, CH., **a, ZY. et al. Crop rotation suppresses tobacco black shank disease incited by Phytophthora nicotianae and influenced the structure of rhizosphere bacterial communities. J Plant Pathol 105, 673–682 (2023). https://doi.org/10.1007/s42161-023-01411-3
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DOI: https://doi.org/10.1007/s42161-023-01411-3