Abstract
A phytoplasma strain associated with pot marigold phyllody disease in Fars province of Iran was identified and characterized by molecular analyses and biological studies. Nested polymerase chain reaction using universal P1/P7 primer pair followed by R16F2n/R2, confirmed the presence of phytoplasmas in the symptomatic plants only, and in Psammotettix striatus leafhopper. BLAST search, phylogenetic analysis, and virtual RFLP patterns of cloned amplicons allowed to classify the pot marigold phyllody and P. striatus leafhopper phytoplasmas in the 16SrI-S subgroup. The phytoplasma was transmitted from infected pot marigold to periwinkle plants by dodder inducing symptoms typical of phytoplasma presence. P. striatus transmitted this phyllody phytoplasma to healthy pot marigold and periwinkle plants after its collection in the infected fields or after acquisition feeding on infected pot marigold plants under insect-proof greenhouse conditions. This is the first report of 16SrI-S phytoplasma associated with phyllody of pot marigold and of a phytoplasma of this ribosomal subgroup in Iran.
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The authors gratefully acknowledge the Plant Protection Research Department of Fars (Iran) Agricultural and Natural Resources centre for supporting this work.
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Salehi, E., Salehi, M., Faghihi, M.M. et al. Molecular characterization and transmission of a 'Candidatus Phytoplasma asteris' strain associated with pot marigold phyllody in Iran. J Plant Pathol 104, 1457–1464 (2022). https://doi.org/10.1007/s42161-022-01229-5
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DOI: https://doi.org/10.1007/s42161-022-01229-5