Abstract
RNA silencing is a conserved and specific degradation mechanism of a target RNA by virus-derived small interfering RNAs in eukaryotic organisms. Identification of viruses of Alcea rosea by small RNA deep sequencing showed the presence of watermelon mosaic virus, malva vein clearing virus, and a putative new closterovirus, which was provisionally named Alcea rosea virus 1 (ArV1). To clarify its taxonomic position, we cloned and analyzed the entire genome of the novel virus. The complete genome of ArV1 has a size of 15318 nt, and contains nine open reading frames (ORFs), namely, a large ORF encoding a putative viral polyprotein 1a (ORF1a), an RNA-dependent RNA polymerase (ORF1b), a p7 (ORF2), a heat shock protein 70 homolog (HSP70h, ORF3), a HSP-90-like protein (ORF4), a coat protein minor (ORF5), a coat protein (ORF6), a p20 (ORF7), and a p21 (ORF8) in the 5′ to 3′ direction. ArV1 shared nucleotide sequence identities of 30.69%–62.62% with 38 other completely sequenced closteroviruses. The amino acid sequence identities between ArV1 and closteroviruses were 34.57%–58.67%. Phylogenetic analysis of HSP70h sequences placed the new virus with members of genus Closterovirus. These results indicate that ArV1 is a member of a new species of the genus Closterovirus.
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (Grant No. 31601612 and Grant No. 31772130), and Natural Science Foundation of Shanxi Province of China (Grant No. 201701D221159).
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Defu Wang and Liyan Cui should be considered co-first authors
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Wang, D., Cui, L., Yu, P. et al. A new closterovirus in Alcea rosea identified by small RNA deep sequencing. J Plant Pathol 102, 29–37 (2020). https://doi.org/10.1007/s42161-019-00369-5
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DOI: https://doi.org/10.1007/s42161-019-00369-5