Abstract
A novel underground reservoir was constructed in an abandoned tunnel for preserving the water in a remote island. The microbial community, potential opportunistic pathogens (OPs) and antibiotic resistance genes (ARGs) of water were analyzed over a year. Results demonstrated that the culturability of the total bacteria and coliforms were both sensitive to the temperature, and the total bacteria culturability had significantly positive relationship with temperature over a year. The ∑ARGs abundance sharply decreased from September to January, but bounced back in July, which was strongly related with phylum Proteobacteria, genera Methylophilus and Methylotenera. The potential OPs almost carried a variety of ARGs, especially in genera Pseudomonas, Enterobacter, Klebsiella, Escherichia with more than 40 ARGs types, which resulted in ARGs enrichment and transfer in the water system. The ∑ARGs of potential OPs variation was extremely significantly related with ARGs subtypes of MacB (r = 0.980, p < 0.05), AcrB (r = 0.997, p < 0.01), AcrA (r = 0.986, p < 0.05) and genus Klebsiella (r = 0.962, p < 0.05).
Article Highlights
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Significantly positive relationship between total bacteria number and temperature.
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∑ARGs strongly related with phylum Proteobacteria, genera Methylophilus/Methylotenera.
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OPs of Pseudomonas, Enterobacter, Klebsiella, Escherichia with > 40 ARGs subtypes.
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∑ARGs of potential OPs extremely significantly related with genus Klebsiella.
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The work was supported by Demonstration Project of Ministry of Water Resources (SF-201817).
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Weng, Y., Qiao, Y., **ao, C. et al. Seasonal Variation of Microbial Community, Potential Opportunistic Pathogens and Antibiotic Resistance Genes in a Novel Underground Reservoir of an Island. Int J Environ Res 16, 59 (2022). https://doi.org/10.1007/s41742-022-00435-4
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DOI: https://doi.org/10.1007/s41742-022-00435-4