Abstract
The activity and expression level of cytoplasmic malate dehydrogenase (MDH 1.1.1.37) gene and adenylate system state in tissues of the Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 were investigated. The highest values of adenylate energy charge (AEC) (0.66 ± 0.01) and adenylate content (ATP, ADP, and AMP) were observed in the mollusc’s foot. Close AEC values were found in the gills (0.61 ± 0.02). The lowest values were recorded in the hepatopancreas and mantle edge (AEC of 0.46–0.54) indicating moderate depression of physiological processes. A direct relationship between adenylate system state in muscle tissue and MDH activity (R2 above 0.7) was revealed, and thus this parameter can be recommended as a biomarker for energy status assessment in the mollusc’s tissue structures. A direct relationship between gene expression levels and MDH activity was shown for tissues of mussels in the range hepatopancreas → gills → foot. In contrast, in the mantle high levels of gene expression corresponded to a relatively low activity of the enzyme, presumably due to non-homogeneous tissue structure.
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Acknowledgements
State assignment No 121041400077-1; Russian Foundation for Basic Research (grant No 20-04-00037 A); Strategic project No 3, No 121121700318-1.
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Aleksander A. Soldatov: Conceptualization, Supervision, Writing – review & editing, Funding acquisition, Project administration, Formal analysis, Data curation. Irina V. Golovina: Determination of MDР activity. Anastasia O. Lantushenko and Alyona S. Kokhan: Determination of MDG gene expression level. Inna V. Sysoeva and Aleksander A. Sysoev: Assessment of the state of the adenylate system. Evgenia E. Kolesnikova: Translation, Review & editing.
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All procedures using mussels were accomplished in accordance with the European Communities Council Directive (2010/63/EU) and approved by the local Institutional Animal Care and Use Committee (protocol #28 from 15.02. 2018).
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Soldatov, A.A., Golovina, I.V., Lantushenko, A.O. et al. Adenylate System State, Malate Dehydrogenase Activity and Expression Level in Tissues of Mytilus galloprovincialis Lamarck, 1819. Thalassas 39, 739–747 (2023). https://doi.org/10.1007/s41208-023-00563-4
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DOI: https://doi.org/10.1007/s41208-023-00563-4