SpringerLink
Log in

Synthesis, characterization of silver nanoparticles from Punica granatum L. and its in vitro antidiabetic activity

  • Original Paper
  • Published:
Nanotechnology for Environmental Engineering Aims and scope Submit manuscript

Abstract

Ecofriendly nanoparticle synthesis is an ongoing worldwide effort to treat many human diseases. In the present study, Punica granatum peel extract is a reducing agent for green synthesizing silver nanoparticles (AgNPs). Further partially characterized by preliminary phytochemical screening, UV–visible spectroscopy analysis, Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography–mass spectroscopy analysis (GC–MS). AgNPs potential was confirmed by in vitro antidiabetic activity, and its ability to inhibit carbohydrate digesting enzymes. AgNPs showed the least inhibition of enzymes with the highest value of 84.0% at 20 mg/ml, 79.0% at 20 mg/ml concentration of α-amylase and α-glucosidase enzyme activity, respectively. The enzymatic assay results revealed the existence of AgNPs can exhibit the dose-dependent inhibition on α Amylase enzyme α-glucosidase potentials. Our results suggest that P. granatum peel extract contains (AgNPs) a good antidiabetic agent against the key enzymes involved in diabetes. Hence, moral activity can be used to make appropriate nanomedicines and pharmaceutical industries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Min Y, Caster JM, Eblan MJ, Wang AZ (2015) Clinical translation of nanomedicine. Chem Rev 115:11147–11190

    Article  Google Scholar 

  2. Ge L, Li Q, Wang M, Ouyang J, Li X, **ng MMQ (2014) Nano silver particles in medical applications: synthesis, performance, and toxicity. Int J Nanomed 9:2399–2407

    Google Scholar 

  3. Mirza AZ, Siddiqui FA (2014) Nanomedicine and drug delivery: a mini review. Int Nano Lett 4:94–99

    Article  Google Scholar 

  4. Uchegbu IF, Schatzlein AG (2010) Burger’s medicinal chemistry, drug discovery and development, 7th edn. Wiley, Hoboken

    Google Scholar 

  5. Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE (2014) Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Res Clin Pract 103:137–149

    Article  Google Scholar 

  6. Exteberria U, De La Garza AL, Campin J, Martínez JA, Milagro FI (2012) Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylase. Expert Opin Ther Targets 16:269–297

    Article  Google Scholar 

  7. Albrecht M, Jiang W, Kumi-Diaka J, Lansky EP, Gommersall LM, Patel A, Mansel RE, Neeman I, Geldof AA, Campbell MJ (2004) Pomegranate extracts potently suppress proliferation, xenograft growth and invasion of human prostate cancer cells. J Med Food 7:274–283

    Article  Google Scholar 

  8. Jainendra Kumar B, Narender B, Vasudha B (2017) Phytochemical screening and evaluation of anti-emetic activity of Punica granatum leaves. Eur J Pharm Med Res 4:526–532

    Google Scholar 

  9. Howell AB, D’Souza DH (2013) The pomegranate: effects on bacteria and viruses that influence human health. Evid-Based Compl Altern Med 60:212–221

    Google Scholar 

  10. Lansky EP, Newman RA (2007) Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol 109:177–206

    Article  Google Scholar 

  11. Sharma P, McClees SF, Afaq F (2017) Pomegranate for prevention and treatment of cancer: an update. Molecules 22:177–182

    Article  Google Scholar 

  12. Amakura Y, Okada M, Tsuji S, Tonogai Y (2000) High-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits. J Chromatogr A 896:87–93

    Article  Google Scholar 

  13. Al-Zoreky NS (2009) Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. Int J Food Microbiol 134:244–248

    Article  Google Scholar 

  14. Khan SA (2009) The role of pomegranate (Punica granatum L.) in colon cancer. Pak J Pharm Sci 22:346–348

    Google Scholar 

  15. Yousef MS, Abdelhamid HN, Hidalgo M, Fathy R, Gómez-Gascón L, Dorado J (2021) Antimicrobial activity of silver-carbon nanoparticles on the bacterial flora of bull semen. Theriogenology 161:219–227

    Article  Google Scholar 

  16. Debiyi OO, Sofowora FA (1978) Phytochemical screening of medical plants. Ilyoidia 3:234–246

    Google Scholar 

  17. Mubail A, Chatterji S, Rai PM, Watal G (2012) Evidence based green synthesis of nanoparticles. Adv Mater Lett 3:519–525

    Article  Google Scholar 

  18. Basu A, Penugonda K (2009) Pomegranate juice: a heart-healthy fruit juice. Nutr Rev 67:49–56

    Article  Google Scholar 

  19. Raskin I, Ribnicky DM, Komarnytsky S, Llic N, Poulev A, Borisjuk N, Brinker A, Moreno DA, Ripoll C, Yakoby N (2002) Plants and human health in the twenty-first century. Trends Biotechnol 20:522–531

    Article  Google Scholar 

  20. Kumar A, Vijayalakshmi K (2013) In vitro antimicrobial activity & phytochemical analysis of selected fruit wastes. Int J Curr Microbiol Appl Sci 2:196–204

    Google Scholar 

  21. Tahsin B, Vaishali J, Geetanjali TB, Zinjarde S (2020) Gold nanoparticles biosynthesized by Nocardiopsis dassonvillei NCIM 5124 enhance osteogenesis in gingival mesenchymal stem cells. Appl Microbiol Biotechnol 104:4081–4092

    Article  Google Scholar 

  22. Etxeberria U, De La Garza AL, Campin J, AlfredoMartínez J, Fermín IM (2012) Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylase. Expert Opin Ther Targets 16:269–297

    Article  Google Scholar 

  23. Nickavar B, Abolhasani L (2013) Bioactivity-guided separation of an α-amylase inhibitor flavonoid from Salvia virgata. Iran J Pharm Res 12:57–61

    Google Scholar 

  24. Banihani S, Swedan S, Alguraan Z (2013) Pomegranate and type 2 diabetes. Nutr Res 33:341–348

    Article  Google Scholar 

Download references

Acknowledgements

The authors are thankful to the authority of Idhaya College for Women, National College (Autonomous) and Annamalai University for providing necessary facilities and support.

Author information

Authors and Affiliations

  1. Department of Biochemistry, Idhaya College for Women, Sarugani, Tamil Nadu, 630411, India

    Usharani Saminathan & Anjalidevi Chinathambi

  2. Department of Biotechnology and Microbiology, National College (Autonomous), Tiruchirapalli, Tamil Nadu, 620001, India

    Pasiyappazham Ramasamy

  3. Department of Microbiology, Faculty of Science, Annamalai University, Chidambaram, Tamil Nadu, 608002, India

    Sivagurunathan Paramasivam, Sumathi Vadamalai & Uma Chinnaiyan

  4. Department of Biotechnology, Rajah Serfoji Government College (Autonomous), Thanjavur, Tamil Nadu, 613005, India

    Rajesh Singh

Authors
  1. Usharani Saminathan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pasiyappazham Ramasamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anjalidevi Chinathambi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sivagurunathan Paramasivam
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sumathi Vadamalai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Uma Chinnaiyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rajesh Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Usharani Saminathan.

Ethics declarations

Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Humans and animals rights

This manuscript does not contain research involving humans and animals.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Saminathan, U., Ramasamy, P., Chinathambi, A. et al. Synthesis, characterization of silver nanoparticles from Punica granatum L. and its in vitro antidiabetic activity. Nanotechnol. Environ. Eng. 7, 923–930 (2022). https://doi.org/10.1007/s41204-022-00278-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s41204-022-00278-6

Keywords

Search

Navigation