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Chlamydomonas as a model system for studying the relevance of plant genes for their involvement in multiple individual and combined stresses: a study unravelling PgCuZnSOD and PgAPX gene functions

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Abstract

The reactive oxygen species (ROS) including superoxide (O2−), hydrogen peroxide (H2O2), and singlet oxygen (O2−), are the main molecules produced in excessive amounts under stress conditions, leading to cellular damage in plants. These ROS molecules should be effectively removed to impart stress tolerance. Enzymatic scavengers like superoxide dismutase, catalase, and peroxidases plays a significant role in scavenging ROS molecules. However, the functional validation of such genes cloned from hardy crops are very much limited. The present research demonstrates the synergistic effect of co-expressing multiple antioxidant genes encoding copper-zinc superoxide dismutase (Cu/Zn-SOD) and ascorbate peroxidase cloned from hardy crop Pennisetum glaucum to improve single and combined abiotic stress tolerance and also corroborates Chlamydomonas as a valuable model system for functional validation of multi genes, especially for understanding combined stress responses due to its rapid transformation process and molecular similarity to higher plants.

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All data generated and analyzed during this study are included in this article.

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Acknowledgements

The authors sincerely thank Dr. MK Reddy, ICGEB, New Delhi for providing the gene construct and the late Dr. M. Udayakumar for his guidance and support during research.

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Author notes

  1. H. M. Mamrutha and Geetha Govind have contributed equally to this work.

Authors and Affiliations

  1. ICAR-Indian Institute of Wheat and Barley Research, Agrasain Marg, Karnal, 132001, Haryana, India

    H. M. Mamrutha

  2. Department of Crop Physiology, Agricultural College, Hassan, 573 225, Karnataka, India

    Geetha Govind

  3. Department of Crop Physiology, University of Agricultural Sciences (UAS), GKVK, Bangalore, 560 065, India

    Hema Ramanna & Karaba N. Nataraja

  4. Department of Plant Protection Biology, Swedish University of Agricultural Sciences, 23422, Lomma, Sweden

    Radha Sivarajan Sajeevan

  5. ICAR-Indian Institute of Horticultural Research, Hesarghatta Lake Post, Bangalore, 560089, India

    A. N. Lokesha

  6. National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Muthappa Senthil-Kumar

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  1. H. M. Mamrutha
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Contributions

HMM and NNK conceived and designed the research with HR. HMM, GG, SRS, and LAN conducted the experiments. HMM, GG, SM, SRS and LAN wrote the manuscript. MS-K and NK edited the manuscript. All authors read and approved the manuscript for publication.

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Correspondence to H. M. Mamrutha or Karaba N. Nataraja.

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Mamrutha, H.M., Govind, G., Ramanna, H. et al. Chlamydomonas as a model system for studying the relevance of plant genes for their involvement in multiple individual and combined stresses: a study unravelling PgCuZnSOD and PgAPX gene functions. Plant Physiol. Rep. 29, 176–185 (2024). https://doi.org/10.1007/s40502-023-00765-1

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