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Radio-sensitivity of diverse tomato genotypes with respect to optimization of gamma irradiation dose

  • Genetics & Evolutionary Biology - Original Article
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Abstract

Genetic variation of cultivated tomato can be enhanced by induced mutagenesis. Multivariate analysis based on 10 important fruit yield components grouped 21 tomato genotypes into 10 clusters indicating the presence of ample diversity among the genotypes. The genotypes Alisa Craig Aft and CLN-B showed best performance. Seven doses of gamma radiation was employed by imposing 100 Gy–700 Gy radiation with 100 Gy interval to the dry seeds of 5 diverse genotypes selected from different clusters which included the best performers Alisa Craig Aft, CLN-B, two wild relatives Solanum pimpinellifolium L., Solanum lycopersicum var. cerasiformae (Dunal) Spooner and an induced mutant Pmut-5. Seedling emergence and shoot length of these genotypes were recorded to determine the median lethal dose (LD50) and median growth reduction dose (GR50). Probit analysis suggested linear decrease in seed germination and shoot length with the increase in the dose of gamma irradiation for all the five genotypes. The LD50 and GR50 values varied widely among the genotypes, being very high for the wild relatives S. lycopersicum var. cerasiformae (298 Gy and 300 Gy) and S. pimpinellifolium (288 Gy and 295 Gy) followed by 240 Gy 251 Gy for Pmut-5, 179 Gy and 186 Gy for Alisa Craig Aft and 173 Gy 182 Gy for CLN-B. The study suggested the optimum gamma radiation dose ranged between 150 and 300 Gy. This optimum dose can be used to induce a higher rate of effective mutation creating maximum favorable genetic diversity with little biological damage in M2 and succeeding generations of tomato.

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Acknowledgements

We thank the Regional Nuclear Agricultural Research Centre (RNARC), Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal, India, for letting us use the facility for this investigation.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. School of Agricultural Sciences, JIS University, Kolkata, West Bengal, India

    Soham Hazra

  2. Department of Genetics and Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, 741252, India

    Soham Hazra, Shouvik Gorai, Sudip Bhattacharya & Anirban Maji

  3. Department of Vegetable Science, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India

    Suvojit Bose, Pranab Hazra & Arup Chattopadhyay

Authors
  1. Soham Hazra
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  2. Shouvik Gorai
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  3. Sudip Bhattacharya
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  4. Suvojit Bose
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  5. Pranab Hazra
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  6. Arup Chattopadhyay
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  7. Anirban Maji
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Contributions

All authors contributed significantly towards the final make-up of the paper.

Conceptualisation (PH and SH); Data curation (SH and SB); Formal analysis (SG and SB); Investigation and methodology (SH and SG); Supervision (PH and AC); Writing-original draft (SH and PH); Writing-reviewing and editing (AM and AC).

Corresponding author

Correspondence to Shouvik Gorai.

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Hazra, S., Gorai, S., Bhattacharya, S. et al. Radio-sensitivity of diverse tomato genotypes with respect to optimization of gamma irradiation dose. Braz. J. Bot 45, 917–927 (2022). https://doi.org/10.1007/s40415-022-00823-2

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  • DOI: https://doi.org/10.1007/s40415-022-00823-2

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