Abstract
Background
Type 2 diabetes mellitus (T2DM) is a complex metabolic disease that occurs as a result of insulin resistance and low insulin production. T2DM involves many organ systems that include macro-vascular and micro-vascular complications. Several genome-wide association studies (GWAS) and candidate gene studies have suggested a large number of single nucleotide polymorphisms (SNPs) on several genes such as HHEX that were associated with T2DM susceptibility. The current study aims to look at the relationship between the risk of T2DM and the HHEX gene variant rs7923837.
Methods
In this case-control study genoty** of rs7923837 of the HHEX gene was performed using the PCR-RFLP and Sanger sequencing method.
Results
Frequencies of GG genotype of rs7923837 polymorphism of HHEX among subjects with and without diabetes mellitus were 33.77% and 25.47% respectively. Corresponding prevalence for the AG genotype was 51.08% and 64.15% among subjects with and without diabetes mellitus respectively. The differences were not statistically significant (p = 0.08).
Conclusion
Our study revealed that polymorphisms rs7923837 of HHEX were not associated with T2DM.
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Data availability
This project has no associated data to be available.
Abbreviations
- T2DM:
-
type 2 diabetes mellitus
- GWAS:
-
genome-wide association studies
- HHEX:
-
hematopoietically expressed homeobox
- SNPs:
-
single nucleotide polymorphisms
- FPG:
-
fasting plasma glucose
- HbA1c:
-
hemoglobin A1c
- OGTT:
-
oral glucose tolerance test
- ADA:
-
American Diabetes Association
- PCR:
-
polymerase chain reaction
- IDF:
-
International Diabetes Federation
- DR:
-
diabetic retinopathy
- DN:
-
diabetic nephropathy
- OR:
-
odds ratio
- CI:
-
confidence interval
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Acknowledgements
The authors would like to express their gratitude to T2DM patients for their participation in this study.
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MH managed the project; SH drafted the manuscript and performed laboratory methods; MA performed the statistical analysis; MM, FAM, and HRAM: provided clinical guidance to the research. All authors contributed to and approved the final version of the manuscript.
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All patients signed written consent forms and the study was approved by the ethical committee (Ethic Code: IR.TMU.REC.1395.19).
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Hasti, S., Mirfeizi, M., Afshari, M. et al. Association of common single-nucleotide polymorphism of HHEX with type 2 diabetes mellitus. J Diabetes Metab Disord 23, 1183–1187 (2024). https://doi.org/10.1007/s40200-024-01407-5
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DOI: https://doi.org/10.1007/s40200-024-01407-5