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Identification, Characterization, Isolation, and Pathogenicity Study of Ralstonia solanacearum: Foundation for Development of a Highly Selective DNA-Biosensing Platform

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Biosensing diagnostic tools require biorecognition elements to precisely identify the causative pathogen, and the effectiveness of these devices is highly dependent on their biorecognition capabilities. In this study, we demonstrate the identification of a highly specific single-stranded DNA probe sequence (lpxC4) to be used as a universal biorecognition element toward the development of biosensing platforms for early detection of a soil-borne agricultural pathogen Ralstonia solanacearum. We have collected and studied 8 isolates of R. solanacearum infecting solanaceous vegetables from different parts of India as potential targets of this biorecognition element. The pathogenicity study on these isolates revealed their disease-causing abilities on eggplant. The extracted genomic DNA from the isolates were amplified using polymerase chain reaction and stored as the potential targets for further studies. Isolation of DNA targets from multiple strains and the identified DNA probe served as the foundation for develo** various biosensing platforms for detecting plant pathogens.

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Acknowledgements

This work was financially supported by the India–Trento Program for Advanced Research (ITPAR—IV) joint project in the field of agricultural research through Department of Science and Technology (DST), Government of India. The first author would like to thank DST, Government of India, for the Ph.D. INSPIRE fellowship.

Funding

DST INSPIRE, Government of India, IF180964, Rhea Patel, ITPAR 4, DST, India.

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Authors and Affiliations

  1. Centre for Research in Nanotechnology and Science, Indian Institute of Technology Bombay, Mumbai, 400076, India

    Rhea Patel

  2. Department of Electrical Engineering, Indian Institute of Technology Bombay, Mumbai, 400076, India

    Madhuri Vinchurkar, Rajul Patkar & Maryam Shojaei Baghini

  3. Plant Pathology, ICAR-Central Coastal Agricultural Research Institute, Old Goa, Goa, 403402, India

    Raman Ramesh

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  1. Rhea Patel
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  2. Madhuri Vinchurkar
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  4. Raman Ramesh
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  5. Maryam Shojaei Baghini
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Correspondence to Rhea Patel.

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Significance Statement: Identification of a highly specific single-stranded DNA probe sequence (lpxC4) to be used as a universal biorecognition element towards the development of biosensing platforms for early detection of a soil-borne pathogen Ralstonia solanacearum. We have studied 8 isolates of R. solanacearum from different parts of India as potential targets.

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Patel, R., Vinchurkar, M., Patkar, R. et al. Identification, Characterization, Isolation, and Pathogenicity Study of Ralstonia solanacearum: Foundation for Development of a Highly Selective DNA-Biosensing Platform. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 93, 595–601 (2023). https://doi.org/10.1007/s40011-023-01464-5

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  • DOI: https://doi.org/10.1007/s40011-023-01464-5

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