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The Effects of Injectable Platelet-Rich Fibrin and Advanced-Platelet Rich Fibrin on Gingival Fibroblast Cell Vitality, Proliferation, Differentiation

  • Original Article
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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Injectable Platelet Rich Fibrin (I-PRF) and Advanced-Platelet Rich Fibrin (A-PRF) are autologous materials derived from patients’ blood and employed in periodontal regenerative surgery. Although I-PRF and A-PRF have different characteristics, their biological effects on gingival tissue fibroblasts remain unclear. This research aims to compare the in vitro capacity in inducing gene expression and proliferation of human gingival fibroblasts between A-PRF and I-PRF.

Methods:

Human donors undergoing dental implant surgery were sampled for normal human gingival fibroblasts (NHGFCs), followed by preparing A-PRF and I-PRF membranes. Enzyme-linked immunosorbent assay (ELISA) kit was used to assess the release of platelet-derived growth factor-AA (PDGF-AA), transforming growth factor-beta1 (TGF- β1), and insulin growth factor-1 (IGF-1) at different periods. Cell viability and proliferation of A-PRF and I-PRF were compared using CCK-8 assay. The impacts of platelet concentration on human gingival fibroblast cells (HGFCs) were evaluated by quantifying the level or amount of phosphorylated extracellular signal-regulated protein kinase (p-ERK), and Matrix metalloproteinases (MMPs), MMP-1 and MMP-3. The effects of PRF on aged human gingival fibroblast cells were examined retrospectively.

Results:

Overall, A-PRF demonstrated a higher release of TGF-B1 and PDGF-AA, while I-PRF reflected higher levels of IGF-1. A significantly higher level of cell proliferation was induced by higher cell proliferation by A-PRF and I-PRF. Additionally, in comparison to I-PRF, the expression of ERK phosphorylation and MMP-1 &MMP-3 in HGFCs was demonstrated by I-PRF and A-PRF. The increase in A-PRF was time-dependent (p < 0.05).

Conclusion:

Both I-PRF and A-PRF induced a stimulatory biological impact on the proliferation of human gingiva fibroblasts, with the latter demonstrating better capacity in facilitating the release of different growth factors. A-PRF also induced higher gene expression of p-ERK, MMP-1 &MMP-3, and the proliferation of fibroblasts.

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Data availability statement

All relevant study data are available in the tables and figures of the manuscript. If you need more details, please contact the corresponding author.

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Acknowledgements

This research was funded by the 1) Lanzhou Talent Innovation and Venture Project (2017-RC-31), 2) Competitive Projects for Science and Technology Innovation and Development of Gansu Province (2018ZX-10), and 3) Gansu Key Technologies R&D Program for International S&T Cooperation and Exchange (18YF1WA116).

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Authors and Affiliations

  1. Department of Oral Implantology, School/Hospital of Stomatology, Lanzhou University, Lanzhou, Gansu, 730000, China

    Sarraj H. Ashour, Omar A. Al-Aroomi, Reem Al-Attab, Wanxin Li & Lihua Yin

  2. Department of Oral and Maxillofacial Surgery and Periodontology, Faculty of Dentistry, The Arab American University, Jenin, 240, Palestine

    Mahmoud Mudalal

  3. Department of Oral Implantology, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, China

    Omar A. Al-Aroomi

Authors
  1. Sarraj H. Ashour
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  3. Omar A. Al-Aroomi
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  4. Reem Al-Attab
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  5. Wanxin Li
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Contributions

Conceptualization: LY, SHA, and MM; methodology: SHA, and MM; software: RA-A, OAA-A; validation: OAA-A; formal analysis: LY; investigation: MM; resources, and data curation: SHA and LY; writing—original draft preparation: SHA; writing—review and editing: MM, OAA-A, and WL; supervision: LY and WL; project administration: OAA-A, RA-A; funding acquisition: LY.

Corresponding author

Correspondence to Lihua Yin.

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The authors have no competing interests to disclose.

Ethical statement

The study was approved by the Ethics Committee of the School/Hospital of Stomatology, Lanzhou University, China (LZUKQ-2022-030).

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Ashour, S.H., Mudalal, M., Al-Aroomi, O.A. et al. The Effects of Injectable Platelet-Rich Fibrin and Advanced-Platelet Rich Fibrin on Gingival Fibroblast Cell Vitality, Proliferation, Differentiation. Tissue Eng Regen Med 20, 1161–1172 (2023). https://doi.org/10.1007/s13770-023-00586-1

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