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Comparison of the Infant and Adult Adipose-Derived Mesenchymal Stem Cells in Proliferation, Senescence, Anti-oxidative Ability and Differentiation Potential

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Infant adipose-derived mesenchymal stem cells (ADSCs) collected from excised polydactyly fat tissue, which was surgical waste, could be cultured and expanded in vitro in this study. In addition, the collecting process would not cause pain in the host. In this study, the proliferation, reduction of senescence, anti-oxidative ability, and differentiation potential in the infant ADSCs were compared with those in the adult ADSCs harvested from thigh liposuction to determine the availability of infant ADSCs.

Methods:

Proliferation was determined by detecting the fold changes in cell numbers and doubling time periods. Senescence was analyzed by investigating the age-related gene expression levels and the replicative stress. The superoxide dismutase (SOD) gene expression, adipogenic, neurogenic, osteogenic, and tenogenic differentiation were compared by RT-qPCR. The chondrogenic differentiation efficiency was also determined using RT-qPCR and immunohistochemical staining.

Results:

The proliferation, SOD (SOD1, SOD2 and SOD3) gene expression, the stemness-related gene (c-MYC) and telomerase reverse transcriptase of the infant ADSCs at early passages were enhanced compared with those of the adults'. Cellular senescence related genes, including p16, p21 and p53, and replicative stress were reduced in the infant ADSCs. The adipogenic genes (PPARγ and LPL) and neurogenic genes (MAP2 and NEFH) of the infant ADSC differentiated cells were significantly higher than those of the adults’ while the expression of the osteogenic genes (OCN and RUNX) and tenogenic genes (TNC and COL3A1) of both demonstrated opposite results. The chondrogenic markers (SOX9, COL2 and COL10) were enhanced in the infant ADSC differentiated chondrogenic pellets, and the expression levels of SODs were decreased during the differentiation process.

Conclusion:

Cultured infant ADSCs demonstrate less cellular senescence and replicative stress, higher proliferation rates, better antioxidant defense activity, and higher potential of chondrogenic, adipogenic and neurogenic differentiation.

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Acknowledgements

We thank the work that was assisted in part by the Division of the Experimental Surgery of the Department of Surgery, Taipei Veterans General Hospital. This study was supported in part by grants from the Taipei Veterans General Hospital (V107C-108), the Ministry of Science and Technology (MOST 106–2314-B-075–060), the Taoyuan General Hospital, Ministry of Health and Welfare (PTH110024), and the National Yang Ming Chiao Tung University Hospital (RD0021-008).

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Author notes

  1. Szu-Hsien Wu, **-Huei Yu authors have contributed equally to this work.

Authors and Affiliations

  1. Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, 112, Taiwan

    Szu-Hsien Wu

  2. Department of Surgery, School of Medicine, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan

    Szu-Hsien Wu, En-Rung Chiang, Ming-Chau Chang & Jung-pan Wang

  3. Division of Plastic and Reconstructive Surgery, Department of Surgery, School of Medicine, National Defense Medical Center, Taipei, Taiwan

    Szu-Hsien Wu

  4. Department of Orthopedic Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, 33004, Taiwan

    **-Huei Yu

  5. Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, 112, Taiwan

    Yu-Ting Liao, En-Rung Chiang, Ming-Chau Chang & Jung-pan Wang

  6. Department of Orthopaedics, National Yang Ming Chiao Tung University Hospital, Yilan, 260, Taiwan

    Kuo-Hao Liu

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  1. Szu-Hsien Wu
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Correspondence to Jung-pan Wang.

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Ethical statement

The collection of human ADSCs was approved by the Institutional Review Board (IRB) of the Taipei Veterans General Hospital (IRB No. 2020–06-010B), and the adipose tissue was collected with patient informed consent. In addition, the procedures involving animals were approved by the Institutional Animal Care and Use Committee of the Taipei Veterans General Hospital.

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Wu, SH., Yu, JH., Liao, YT. et al. Comparison of the Infant and Adult Adipose-Derived Mesenchymal Stem Cells in Proliferation, Senescence, Anti-oxidative Ability and Differentiation Potential. Tissue Eng Regen Med 19, 589–601 (2022). https://doi.org/10.1007/s13770-022-00431-x

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  • DOI: https://doi.org/10.1007/s13770-022-00431-x

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