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Microbial synthesis of hydroxytyrosol and hydroxysalidroside

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Abstract

Plant-derived phenolic compounds, such as hydroxytyrosol and hydroxysalidroside, have a beneficial impact on human health owing to their antioxidant activity. In this study, we used Escherichia coli to synthesize hydroxytyrosol. Tyrosine decarboxylase from Papaver somniferum, tyrosine oxidase from Micrococcus luteus, and 4-hydroxyphenylacetate 3-monooxygenase from E. coli were transformed into the bacterial cell. The resulting transformant successfully synthesized hydroxytyrosol. Furthermore, we used the engineered E. coli strains to synthesize ~ 268.3 mg/L hydroxytyrosol. Three uridine diphosphate-dependent glycosyltransferases (UGTs), which were previously shown to convert tyrosol into salidroside, were tested to synthesize hydroxysalidroside, and one of UGTs was used to synthesize hydroxysalidroside from hydroxytyrosol. Finally, E. coli harboring this UGT converted approximately 50% of hydroxytyrosol into hydroxysalidroside.

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Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 Program (PJ01326001), Rural Development Administration, and Priority Research Centers Program through the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology (2009-0093824).

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Authors and Affiliations

  1. Department of Integrative Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul, 05029, Republic of Korea

    Hye Jeong Choo, Eun Ji Kim, So Yeon Kim, Youngshim Lee & Joong-Hoon Ahn

  2. Department of Forest Resources, Gyeongnam National University of Science and Technology, 33 Dong**-ro, **ju-si, Gyeongsangnam-do, 52725, Republic of Korea

    Bong-Gyu Kim

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  1. Hye Jeong Choo
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  2. Eun Ji Kim
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  3. So Yeon Kim
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Correspondence to Joong-Hoon Ahn.

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Choo, H.J., Kim, E.J., Kim, S.Y. et al. Microbial synthesis of hydroxytyrosol and hydroxysalidroside. Appl Biol Chem 61, 295–301 (2018). https://doi.org/10.1007/s13765-018-0360-x

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