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Antioxidant and antitumor activities of β-glucan-rich exopolysaccharides with different molecular weight from Paenibacillus polymyxa JB115

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Abstract

β-Glucan isolated from cell wall of fungi not only has low purity and yield, but also causes adverse effects. Consequently, extracellular β-glucan produced by microorganisms is the focus of this study. β-Glucan-rich exopolysaccharides (G-EPSs) with different molecular weights from Paenibacillus polymyxa JB115 were prepared by ultrafiltration and spray-drying. The weight-average molecular masses of the G-EPSs, P-SD-1 (spray-dried G-EPSs powder prepared below 100 kDa) and P-SD-2 (spray-dried G-EPSs powder prepared above 100 kDa), were 7.084×104 and 9.235×105 g/mol, respectively. β-Glucan content was 47.38% in P-SD-1 and 73.12% in P-SD-2. The hydroxyl radical- and superoxide radicalscavenging activities of P-SD-2 at 1 mg/mL (39.45 and 87.34%, respectively) were higher than those of P-SD-1 (30.32 and 53.06%, respectively). Maximal nitric oxide (22.24±1.34 μM) was generated in the presence of P-SD-2 (1 mg/mL) and the antitumor activity of P-SD-2 was higher than P-SD-1 in four tumor cell lines (HeLa, Sarcoma 180, A549, and Hep3B cells). Thus, antioxidant and antitumor activities could be enhanced by regulating the molecular weight of G-EPSs. We anticipate that the food and medicinal use of G-EPSs will follow further characterization of this class of exopolysaccharides.

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Authors and Affiliations

  1. Biohealth Convergence Center, Daegu Technopark, Seong-swo industrial complex-ro, Dalseo-gu, Daegu, Republic of Korea

    Hee Kyoung Jung

  2. Department of Food Science and Technology, Catholic University of Daegu, Geumnakro 5, Hayang-Eup, Gyeongsan-Si, Gyeongbuk, Republic of Korea

    Joo-Heon Hong

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Hong, JH., Jung, H.K. Antioxidant and antitumor activities of β-glucan-rich exopolysaccharides with different molecular weight from Paenibacillus polymyxa JB115. J Korean Soc Appl Biol Chem 57, 105–112 (2014). https://doi.org/10.1007/s13765-013-4252-9

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