Abstract
Total reactive oxygen species (ROS) signals in irradiated Arabidopsis plants were examined by electron paramagnetic resonance (EPR) analysis. At 10 kGy, the EPR signal intensity was highest in the root, whereas relatively low intensity levels were observed in the leaf and stem. The relative unit (r.u.) of control plants was 0.38 in the leaf, which was gradually increased to 0.51, 0.71, and 0.95 r.u. at 1, 5, and 10 kGy, respectively. In the stem, the intensity in all irradiated samples was lowest compared with that in other plant organs such as the leaf and root. The r.u. in the root sharply increased from 0.13 r.u. in control samples to 1.58 r.u. at 10 kGy, with 0.30–0.42 r.u. observed in 1–5 kGy irradiated samples. Stem and leaf extracts showed remarkably high levels of radical scavenging activity at 89.12 and 71.45%, respectively, compared with the very low level of activity in the root at 10.75%. These findings were in good agreement with the extraction yield of each plant organ, which was 20.0, 14.8, and 10.0% in the stem, leaf, and root, respectively. Order of EPR signal intensity and radical scavenging activity was as follows: EPR signal intensity: 1) leaf > root > stem at 1 and 5 kGy, 2) root > leaf > stem at 10 kGy; radical scavenging activity: stem > leaf > root. Results showed high or low levels of EPR signal intensity in different plant organs could be caused by the ROS removal power of extracts from different plant organs.
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References
Brown PD, Tokuhisa JG, Reichelt M, and Gershenzon J (2003) Variation of glucosinolate accumulation among different organs and developmental stages of Arabidopsis thaliana. Phytochemistry 62, 471–481.
Cerutti PA (1985) Peroxidant states and tumor promotion. Science 277, 375–381.
Lee MH, Moon YR, Chung BY, Kim JS, Lee KS, Cho JY, and Kim JH (2009) Practical use of chemical probes for reactive oxygen species produced in biological systems by γ-irradiation. Radiat Phys Chem 78, 323–327.
Leutwiler LS, Hough-Evans BR, and Meyerowitz EM (1984) The DNA of Arabidopsis thaliana. Mol Gen Genet 194, 15–23.
Mensor LL, Menezes FS, Leitao GG, Reis AS, Dos Santos TC, Coube CS, and Leitão SG (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 15, 127–130.
Paktaş DD and Sünetşioğlu MM (2007) EPR spin probe investigation of irradiated wheat, rice and sunflower seeds. Radiat Phys Chem 76, 46–54.
Pedrajas JR, Vizuete AM, Javanmardy N, Gustafsson J (2000) Mitochondria of Saccharomyces cerevisiae contain one-conserved cysteine type peroxiredoxin with thioredoxin peroxidase activity. J Biol Chem 275, 16296–16301.
Polovka M, Brezová V, Staško A, Mazúr M, Suhaj M, and Šimko P (2006) EPR investigations of gamma-irrradiated gound black pepper. Radiat Phys Chem 75, 309–321.
Tomás-Barberán FA and Espín JC (2001) Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables. J Sci Food Agric 81, 853–876.
Yordanova ND, Aleksievaa K, and Mansour I (2005) Improvement of the EPR detection of irradiated dry plantsusing microwave saturation and thermal treatment. Radiat Phys Chem 73, 55–60.
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Lee, M.H., Moon, Y.R., Bai, HW. et al. Electron paramagnetic resonance investigation of different plant organs after gamma irradiation. J Korean Soc Appl Biol Chem 55, 237–240 (2012). https://doi.org/10.1007/s13765-012-1008-x
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DOI: https://doi.org/10.1007/s13765-012-1008-x