Abstract
Moringa oliefera is one of the most important horticultural tree crop with high medicinal value of each of its parts such as leaf, root, stem and pods. DNA based markers especially those based on expressed sequence tag (EST) based simple sequence repeat (SSR) (EST-SSRs) are proven surrogates of difficult-to-select traits in crops including Moringa. A total of 48 polymorphic EST-SSR markers with varying number of repeat motifs were mined from transcripts of leaf, root, stem and pods of a Moringa germplasm accession. These 48 EST-SSR-based markers were validated on a set of 32 genotypes selected based on horticulturally important traits from the germplasm. A total of 36 of the 48 EST-SSR markers successfully amplified with expected range of amplicon size as predicted from in-silico validation. About 485 alleles were detected with a mean of 13.5 alleles. Di- and Tri- nucleotide repeat motif based EST-SSRs exhibited better ability to discriminate the genotypes than those based on other motifs. A perceptibly higher estimates of observed heterozygosity (0.98) than that is expected (0.83) under Hardy- Weinberg equilibrium suggested higher rate of cross-pollination. The accessions could be grouped into three clusters. The pod length of the accessions grouped under the three clusters differed significantly. Considering the discriminating ability of the EST-SSR markers, they can be used in strategic and applied Moringa breeding research.
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Abbreviations
- EST:
-
Expressed sequence tag
- SSR:
-
Simple sequence repeat
- PIC:
-
Polymorphic information content
- MAS:
-
Marker assisted selection
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Acknowledgements
The authors thank ICAR-IIHR, Bengaluru and ICAR for providing the basic infrastructural facilities and funding for successful conducts of the experiment. We would like to thank our Director and Head (Basic Sciences), ICAR-IIHR for their support in this work.
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The research was supported by ICAR-IIHR, Bengaluru.
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Conceptualization and design: KNP, KVR and SR; Material for experiment: RS and KVR; Curation and analysis of data and tabulation of results: KNP and SR; Original draft manuscript writing: KNP and KVR; Editing and communication-ready manuscript version: KNP and SR.
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Poornima, K.N., Shankar, R., Ramesh, S. et al. De-novo development and validation of EST-SSRs in Moringa oliefera. J. Plant Biochem. Biotechnol. 32, 319–327 (2023). https://doi.org/10.1007/s13562-022-00807-z
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DOI: https://doi.org/10.1007/s13562-022-00807-z