Abstract
Vandachostylis ‘Sri-Siam’ is a fragrant orchid, suitable as a potted plant for the floriculture and perfumery industries due to its sweet citrus-like fragrance. Fragrance from the flowers was captured using SPME and subjected to GC–MS analysis. A transcriptome library was constructed from the tepals for sequencing using the Illumina MiSeq Desktop Sequencer and for de novo assembly using the CLC Genomic Workbench version 6.0. All de novo assembly sequences were subjected to bioinformatics analyses including BLASTx analysis, functional annotations and map** of the annotated genes/gene products to the KEGG database using the Blast2go Pro software. GC–MS analysis reveals the fragrance are dominated by 68.73% monoterpenes, 11.26% esters, 4.20% sesquiterpenes and traces of alcohols (1.20%), dissimilar to both its parents. Analysis of the de novo assembly sequences generated 45,585 contigs which identified 14 fragrance-related transcripts. Information on the fragrance constituents and fragrance-related transcripts is deemed important as a reference for current and future work of using this hybrid in the breeding process of commercially valuable fragrant orchids.
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Abbreviations
- BLASTx:
-
Basic local alignment search tool x
- cDNA-RDA:
-
CDNA representational difference analysis
- EC:
-
Enzyme class
- FPP:
-
Farnesyl diphosphate
- GC–MS:
-
Gas chromatography-mass spectrometry
- HMG-coA:
-
3-Hydroxy-3-methylglutaryl-CoA
- KEGG:
-
Kyoto encyclopedia of genes and genomes
- MEP:
-
2-C-methyl-D-erythritol 4-phosphate
- Mrna:
-
Messenger ribonucleic acid
- MVA:
-
Mevalonate
- NCBI:
-
National center for biotechnology information
- NGS:
-
Next generation sequencing
- NIST:
-
National institute of standards and technology
- RACE-PCR:
-
Rapid amplification of cDNA ends polymerase chain reaction
- RNA:
-
Ribonucleic acid
- SPME:
-
Solid phase microextraction
- SRA:
-
Sequence read archive
- TSA:
-
Transcriptome shotgun assembly sequence database
- VSSS:
-
Vandachostylis ‘Sri-Siam
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Acknowledgements
This work was supported by the MOSTI under eScienceFund Grant [02-01-16-SF0049 (RDU120501)]; MOHE under ERGS Grant (RDU110601) and RACE Grant [RDU150305]; and Universiti Malaysia Pahang under Short-term Research Grant [RDU110353]. A special thanks to Dr. Tan Soon Guan, a retired Professor of Genetics, Universiti Putra Malaysia, for his helpful critical review of this paper.
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Mohd-Hairul, A.R., Ong-Abdullah, J., Song, A.AL. et al. Vandachostylis ‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles. J. Plant Biochem. Biotechnol. 31, 965–968 (2022). https://doi.org/10.1007/s13562-022-00783-4
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DOI: https://doi.org/10.1007/s13562-022-00783-4