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Iron (Fe3+)-mediated redox responses and amelioration of oxidative stress in cadmium (Cd2+) stressed mung bean seedlings: a biochemical and computational analysis

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Abstract

The present investigation aims to evaluate the possible ameliorative role of iron (Fe3+) on cadmium (Cd2+)-induced oxidative stress in mung bean (Vigna radiata L.) seedlings using biochemical and computational approaches. Imposition of Cd2+ stress for a period of 7d showed substantial changes in the growth of root and shoot, and was accompanied by significant Cd2+ accumulation and alteration of biochemical parameters. Cd2+ stress resulted in a significant reduction in root and shoot length, biomass and moisture content, and increased the levels of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) and superoxide (O2·−). Furthermore, a significant increase in the rate of lipid peroxidation and degradation of antioxidant defence metabolism were observed. Supplementation of the Cd2+-stressed seedlings with Fe3+ for 7d resulted in amelioration of growth, and restoration of normal cellular homeostasis as observed by considerable changes in oxidative stress parameters as compared to Cd2+ treated seedlings alone. Besides, in absence of Fe3+, Cd2+ accumulation in both root and shoot were significantly high as compared to both control and Fe3+-supplemented seedlings. Computational (molecular docking) analysis with superoxide dismutase and catalase as target enzymes revealed that both Cd2+ and Fe3+ have got affinities for the active sites of these antioxidant enzymes. Thus, it is hereby proposed that Cd2+ may potentially interfere with the activities of these antioxidant enzymes, while Fe3+ may prevent such interactions. Our findings based on biochemical and computational analysis clearly reveals the ameliorative role of Fe3+ on Cd2+-induced alterations in growth and oxidative stress in mung bean seedlings.

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Abbreviations

AsA:

Ascorbate

BHT:

Butylated hydroxytoulene

CAT:

Catalase

DTNB:

5,5′-Dithiobis-2-nitrobenzoic acid

EDTA:

Ethylenediaminetetraacetic acid

GPX:

Guaiacol peroxidase

GR:

Glutathione reductase

GSH:

Glutathione

GSSG:

Oxidised glutathione

KSCN:

Potassium thiocynate

MDA:

Malondialdehyde

MVD:

Molegro Virtual Docker

NADPH:

Nicotinamide adenine dinucleotide phosphate

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

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Acknowledgements

The authors sincerely acknowledges Assam University, Silchar for providing all necessary facilities for this study.

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Author notes

  1. Muhammad Khairujjaman Mazumder and Debojyoti Moulick have contributed equally to this work.

Authors and Affiliations

  1. Plant Stress Biology and Metabolomics Laboratory, Central Instrumentation Laboratory, Assam University, Silchar, 788011, India

    Muhammad Khairujjaman Mazumder, Debojyoti Moulick & Shuvasish Choudhury

  2. Department of Zoology, Dhemaji College, Dhemaji, 787057, India

    Muhammad Khairujjaman Mazumder

Authors
  1. Muhammad Khairujjaman Mazumder
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  2. Debojyoti Moulick
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  3. Shuvasish Choudhury
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Contributions

SC designed, supervised the study, performed the biochemical analysis and wrote part of the manuscript. MKJ and DM conducted the computational (docking) analysis and wrote major part of the manuscript (equal contribution).

Corresponding author

Correspondence to Shuvasish Choudhury.

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Mazumder, M.K., Moulick, D. & Choudhury, S. Iron (Fe3+)-mediated redox responses and amelioration of oxidative stress in cadmium (Cd2+) stressed mung bean seedlings: a biochemical and computational analysis. J. Plant Biochem. Biotechnol. 31, 49–60 (2022). https://doi.org/10.1007/s13562-021-00654-4

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