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The effect of the lignin isolation method from oil palm empty fruit bunch black liquor for seed coating material

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Abstract

Lignin has potency as a seed coating treatment for preserving seed from deterioration prior to plant and prevent seed growth quickly. Nonetheless, given the complexity of lignin characteristics, it is necessary to develop isolation methods to ensure appropriate utilization, particularly for seed coating. This study intended to observe the oil palm empty fruit bunch (OPEFB) lignin for seed coating treatment and evaluate the effect on chili seed growth. Previously, lignin was extracted by inorganic acid, specifically H2SO4 and HCL, via a precipitation method with washing treatment of 3 and 5 times. The effectiveness of lignin as seed coating was evaluated by soaking chili seeds in a lignin-NaOH solution. The results showed that HCl-isolated lignin has a higher purity of 91.62% compared to H2SO4-isolated lignin after 5 washing treatments. Meanwhile, H2SO4-isolated lignin has a higher yield of 19.29%. The syringyl/guiacyl (S/G) ratio of isolated lignin by HCl and H2SO4 with different washing treatments ranges from 0.97 to 1.97. Thermal analysis reveals that HCl-isolated lignin is more stable than H2SO4-isolated lignin, with a mass residue of 49.4%. The glass transition (Tg) ranges from 126.42 to 168.05 °C. The result showed that the shelf life of lignin-coated seed was up to 30 weeks at room temperature and more resistance to fungal contaminants. Germination of chili-coated seeds occurs in the second to fourth weeks after the planting period.

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Acknowledgements

The authors acknowledge the Advanced Characterization Laboratories Cibinong—Integrated Laboratory of Bioproduct (i-Lab), National Research and Innovation Agency, for the facilities.

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Authors and Affiliations

  1. Department of Forest Products, Faculty of Forestry and Environment, IPB University, Bogor, 16680, Indonesia

    Erika Ayu Agustiany & Deded Sarip Nawawi

  2. Research Center for Biomass and Bioproducts, National Research and Innovation Agency (BRIN), Jl. Raya Bogor KM 46, Cibinong, 16911, Indonesia

    Erika Ayu Agustiany, Arief Heru Prianto & Widya Fatriasari

  3. Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Jl. Raya Bogor KM 46, Cibinong, 16911, Indonesia

    Krisna Suzana & Ikhsan Guswenrivo

  4. Department of Biology, Padjadjaran University, Jl. Raya Bandung Sumedang No. Km. 21, Hegarmanah, Jatinangor, Sumedang, West Java, 45363, Indonesia

    Krisna Suzana

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Contributions

Conceptualization: Widya Fatriasari, Ikhsan Guswenrivo, and Deded Sarip Nawawi; methodology: Widya Fatriasari and Erika Ayu Agustiany; software: Erika Ayu Agustiany and Krisna Suzana; validation: Widya Fatriasari and Deded Sarip Nawawi; formal analysis: Widya Fatriasari, Ikhsan Guswenrivo, and Deded Sarip Nawawi; investigation: Widya Fatriasari; resources: Widya Fatriasari and Ikhsan Guswenrivo; data curation: Erika Ayu Agustiany, Ikhsan Guswenrivo, and Widya Fatriasari; writing—original draft preparation: Erika Ayu Agustiany, Ikhsan Guswenrivo, Widya Fatriasari, Deded Sarip Nawawi, and Arief Heru Priyanto; writing—review and editing: Widya Fatriasari, Ikhsan Guswenrivo, and Deded Sarip Nawawi; visualization: Erika Ayu Agustiany and Krisna Suzana; supervision: Widya Fatriasari and Deded Sarip Nawawi; project administration: Widya Fatriasari; funding acquisition: Widya Fatriasari. All authors have read and agreed to the published version of the manuscript.

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Agustiany, E.A., Suzana, K., Guswenrivo, I. et al. The effect of the lignin isolation method from oil palm empty fruit bunch black liquor for seed coating material. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04874-7

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