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Sugarcane bagasse pretreated by different technologies used as support and carbon source in solid-state fermentation by Aureobasidium pullulans LB83 to produce bioemulsifier

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Abstract

Bioemulsifier production by Aureobasidium pullulans LB83 from pretreated sugarcane bagasse was evaluated in solid-state fermentation. Alkaline (0.2 mol.L−1 NaOH; 12 min) and hydrodynamic cavitation (0.5 mol.L−1 NaOH, 25 min) pretreatments showed a maximum lignin removal of 55.1 % and 44.7 %, respectively. Pretreatment biomass effectivity was assessed by X-ray diffraction, FT-MIR, FTIR-NIR, and RAMAN techniques. Maximum kerosene emulsification indexes of 65.8 % and 41.3 % were obtained in solid-state fermentation, respectively, for alkaline and hydrodynamic cavitation pretreated sugarcane bagasse. Synthesis of cellulases was observed in fermentation, showing maximum values of endoglucanase and exoglucanase, respectively, of 2.25 U.g−1 and 1.39 U.g−1, for alkaline pretreated sugarcane bagasse, and 2.43 U.g−1 and 1.45 U.g−1, for hydrodynamic cavitation-pretreated sugarcane bagasse. The biomolecule was characterized as a mixture of mannitol and arabitol-type liamocin. Microorganism was able to produce bioemulsifier using sugarcane bagasse as support and carbon source in solid-state fermentation, thus showing the potential of this system to obtain value-added products from this biomass.

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Acknowledgements

The authors gratefully acknowledge the FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo – Process number 2020/06323-0 and 2016/10636-8) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil) (DS 88882.379239/2019-01) for financial support.

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Funding

This work was supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo – Process number 2020/06323-0 and 2016/10636-8) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Brazil) (DS 88882.379239/2019-01) for financial support.

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Authors and Affiliations

  1. Department of Biotechnology, Engineering School of Lorena, University of São Paulo, Lorena, 12602-810, Brazil

    Rogger Alessandro Mata da Costa, Daylin Rubio-Ribeaux, Bruna Curry Carneiro, Paulo Marcelino Franco, Júlio César dos Santos & Silvio Silvério da Silva

  2. Department of Physics, Federal University of Juiz de Fora, Juiz de Fora, 36036-330, Brazil

    Geissy de Azevedo Mendes, Isis Lee da Silva & Virgílio de Carvalho dos Anjos

  3. iAMB — Institute of Applied Microbiology, ABBt — Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany

    Till Tiso

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  1. Rogger Alessandro Mata da Costa
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Contributions

Rogger Alessandro Mata da Costa: conceptualization, software, formal analysis, investigation, data curation, and writing—original draft preparation. Bruna Carneiro: methodology and validation. Daylin Rubio-Ribeaux: conceptualization and writing—original draft preparation. Paulo Marcelino Franco: conceptualization. Geissy de Azevedo Mendes: formal analysis. Isis Lee da Silva: formal analysis. Virgílio de Carvalho dos Anjos: software and formal analysis. Júlio César dos Santos: supervision and data curation. Till Tiso: supervision. Silvio Silvério da Silva: supervision, resource and funding acquisition.

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Highlights

- Alkaline pretreatment was effective for lignin removal

- Biomass pretreated was used as carbon source for the bioemulsifier production

- Aureobasidium pullulans produced bioemulsifier through solid-state fermentation

- Detection of cellulolytic enzymes confirms the use of sugarcane bagasse as carbon source

- Bioemulsifiers were identified as mannitol and arabitol-type liamocin

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da Costa, R.A.M., Rubio-Ribeaux, D., Carneiro, B.C. et al. Sugarcane bagasse pretreated by different technologies used as support and carbon source in solid-state fermentation by Aureobasidium pullulans LB83 to produce bioemulsifier. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-03896-5

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