Abstract
In this study, we evaluated the effectiveness of thermotherapy in eliminating apple rubbery wood virus 2 (ARWV-2) in six infected apple varieties collected from regions in China. These plants were subjected to high-temperature treatment, followed by excision of shoot tips, which were subsequently grafted onto the rootstock. All varieties exhibited high thermostability, but the survival rates (average: 30.5%) of the shoot tips varied markedly. The regenerated plants were tested using semi-nested PCR; the results showed that the ARWV-2 elimination efficiencies differed greatly among the varieties. The elimination rates were > 80% for Baxianzaofu and Ruixue and < 50% for HL, Longkou no. 3, and Yishuihong. Notably, all surviving Longkou no. 3 plants remained infected with ARWV-2. The average ARWV-2 elimination efficiency was 44.3%, closely aligning with that of the apple stem pitting virus and apple stem grooving virus. This suggests that the presence of ARWV-2 may influence the ability of certain varieties to yield virus-free germplasm. Moreover, analysis of the ARWV-2 sequences from Baxianzaofu, Ruixue, Longkou no. 3, and Yishuihong revealed that the elimination rate exhibited minimal correlation with the virus diversity. The consistency and identity of the different variants within the same isolates or among the four isolates were high. Our findings can serve as a valuable foundation for develo** treatment strategies to address ARWV-2 infections.
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References
Benke AP, Krishna R, Khandagale K, Gawande S, Shelke P, Dukare S, Dhumal S, Singh M, Mahajan V (2023) Efficient elimination of viruses from garlic using a combination of shoot meristem culture, thermotherapy, and chemical treatment. Pathogens 12:129
Bester R, Bougard K, Maree HJ (2022) First report of apple rubodvirus 2 infecting apples (Malus domestica) in South Africa. J Plant Pathol 104:1199–1200
Bettoni JC, Fazio G, Carvalho Costa L, Hurtado-Gonzales OP, Rwahnih MA, Nedrow A, Volk GM (2022) Thermotherapy followed by shoot tip cryotherapy eradicates latent viruses and apple hammerhead viroid from in vitro apple rootstocks. Plants 11:582
Bougard K, Maree HJ, Pietersen G, Meitz-Hopkins JC, Bester R (2022) First report of apple rubodvirus 2 infecting pear (Pyrus communis) in South Africa. Plant Dis 106:772
Clover GRG, Pearson MN, Elliott DR, Tang Z, Smales TE, Alexander BJR (2003) Characterization of a strain of Apple stem grooving virus in Actinidia chinensis from China. Plant Pathol 52:371–378
Fan XD, Dong YF, Zhang ZP, Ren F, Hu GJ, Zhu HJ (2014) Molecular identification and gene sequence analysis of grapevine virus E. Acta Phytopathologica Sinica 44:455–460
Hoffman MT, Doud MS, Williams L, Zhang MQ, Ding F, Stover E, Hall D, Zhang S, Jones L, Gooch M, Fleites L (2013) Heat treatment eliminates ‘Candidatus Liberibacter Asiaticus’ from infected citrus trees under controlled conditions. Phytopathology 103:15–22
Hu GJ, Dong YF, Zhang ZP, Fan XD, Ren F, Zhu HJ (2015) Efficiency of virus elimination from potted apple plants by thermotherapy coupled with shoot-tip grafting. Australas Plant Pathol 44:167–173
Hu GJ, Dong YF, Zhang ZP, Fan XD, Ren F, Li ZN, Zhang SN (2018) Elimination of Grapevine rupestris stem pitting-associated virus from Vitis vinifera ‘Kyoho’by an antiviral agent combined with shoot tip culture. Sci Hortic 229:99–106
Hu GJ, Dong YF, Zhang ZP, Fan XD, Ren F (2019) Elimination of apple necrosis mosaic virus from potted apple plants by thermotherapy combined with shoot-tip grafting. Sci Hortic 252:310–315
Hu GJ, Dong YF, Zhang ZP, Fan XD, Ren F, Lu XK (2021a) First report of apple rubbery wood virus 2 infection of apples in China. Plant Dis 105:519
Hu GJ, Dong YF, Zhang ZP, Fan XD, Ren F, Lu XK (2021b) First report of apple rubbery wood virus 1 in apple in China. Plant Dis 105:3770
Hu GJ, Dong YF, Zhang ZP, Fan XD, Ren F (2022) Inefficiency of Ribavirin to eliminate apple scar skin viroid from apple plants. Plant Cell Tis Org Cult 51:189–197
Jakovljevic V, Otten P, Berwarth C, Jelkmann W (2017) Analysis of the apple rubbery wood disease by next generation sequencing of total rna. Eur J Plant Pathol 148:637–646
Jelkmann W, Keim-Konrad R (1997) Immuno-capture polymerase chain reaction and plate-trapped ELISA for the detection of Apple stem pitting virus. J Phytopathol 145:499–503
Karimpour S, Davarynejad G, Aghl MZ, Safarnejad MR (2020) In vitro thermotherapy and thermo-chemotherapy approaches to eliminate some viruses in Pyrus communis L. Cv. ‘natanz’. J Agr Sci Tech 22:1645–1653
Li LH (1987) Report on the study of virus-free apple seedlings in Northern China. Sichuan Fruit Science and Technology 2:3–7
Li XL, Li MJ, Zhou J, Wei QP, Zhang JK (2020) Acquisition of virus eliminated apple plants by thermotherapy and the factors influenced the eliminating efficiency. Erwerbs-Obstbau 62:257–264
Lim S, Baek D, Moon JS, Cho IS, Choi GS, Do YS, Lee SH (2018) First report of apple luteovirus 1 and apple rubbery wood virus 1 on apple tree rootstocks in Korea. Plant Dis 103:591
Liu L, Chen XQ, Yan LY, ** YK, Sun LL, Yang YZ, Wang YJ, Zhao ZY (2021) Different eradication effects of latent viruses by combining thermotherapy with shoot tip culture or cryotherapy in four apple cultivars. Sci Hortic 288:110356
Minutolo M, Cinque M, Serio FD, Navarro B, Alioto D (2023) Occurrence of apple rubbery wood virus 1 and apple rubbery wood virus 2 in pear and apple in Campania (southern Italy) and development of degenerate primers for the rapid detection of rubodviruses. J Plant Pathol 105:567–572
Navarro B, Zicca S, Minutolo M, Saponari M, Alioto D, Di Serio F (2018) A negative-stranded RNA virus infecting citrus trees: the second member of a new genus within the order Bunyavirales. Front Microbiol 9:2340
Nickel O, Fajardo TVM, Candresse T (2020) First report on detection of three bunya-like viruses in apples in Brazil. Plant Dis 104:3088
Noda H, Yamagishi N, Yaegashi H, **ng F, **e J, Li S, Zhou T, Ito T, Yoshikawa N (2017) Apple necrotic mosaic virus, a novel ilarvirus from mosaic-diseased apple trees in Japan and China. J Gen Plant Pathol 83:83–90
Panattoni A, Triolo E (2010) Susceptibility of grapevine viruses to thermotherapy on in vitro collection of Kober 5BB. Sci Hortic 125:63–67
Rana T, Negi A, Dhir S, Thokchom T, Chandel V, Walia Y, Singh MR, Ram R, Hallan V, Zaidi AA (2011) Molecular diagnosis of apple virus and viroid pathogens from India. Arch Phytopath Plant Prot 44:505–512
Rott M, Kesanakurti P, Berwarth C, Rast H, Boyes I, Phelan J, Jelkmann W (2018) Discovery of negative-sense rna viruses in trees infected with apple rubbery wood disease by next-generation sequencing. Plant Dis 102:1254–1263
Sipahioglu HM, Usta M, Ocakm M (2006) Use of dried high-phenolic laden host leaves for virus and viroid preservation and detection by PCR methods. J Virol Methods 137:120–124
Skiada FG, Maliogka VI, Katis NI, Eleftheriou EP (2013) Elimination of grapevine rupestris stem pitting-associated virus (GRSPaV) from two Vitis vinifera cultivars by in vitro chemotherapy. Eur J Plant Pathol 135:407–414
Wang QC, Cuellar WJ, Rajamäki M, Hirata Y, Valkonen JPT (2008) Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Mol Plant Pathol 8:1–14
Wang Y, Wang GP, Hong N, Wang YX, Yang ZK, Guo JS, Qi LY (2019) First report of apple rubbery wood virus 2 infecting pear (Pyrus spp.) in China. Plant Dis 103:3293
Wang Y, Wang Y, Wang G, Li Q, Zhang Z, Li L, Lv Y, Yang Z, Guo J, Hong N (2022) Molecular characteristics and incidence of apple rubbery wood virus 2 and citrus virus a infecting pear trees in China. Viruses 14:576
Wright AA, Szostek SA, Beaver-Kanuya E, Harper SJ (2018) Diversity of three bunya-like viruses infecting apple. Arch Virol 163:3339–3343
Zambrana-Echevarria C, Roth M, Dasgupta R, German T, Smith DL (2021) Sensitive and specific qPCR and nested RT-PCR assays for the detection of tobacco streak virus in soybean. Plant Dis 104:521–526
Zhang SN, Li ZN, Fan XD, Zhang ZP, Ren F, Hu GJ, Dong YF (2018) Establishment of RT-LAMP assay for detection of apple chlorotic leaf spot virus (ACLSV). Scientia Agricultura Sinica 51:1706–1716
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This work was financially supported by Applied Basic Research Program of Liaoning Province (2022JH2/101300189) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.
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Hu, GJ., Dong, YF., Zhang, ZP. et al. Efficiency of thermotherapy for eliminating apple rubbery wood virus 2 and other viruses from apple plants. Australasian Plant Pathol. 53, 151–157 (2024). https://doi.org/10.1007/s13313-024-00966-8
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DOI: https://doi.org/10.1007/s13313-024-00966-8