Abstract
Anthracnose, caused by Colletotrichum gloeosporioides complex, is an important disease of strawberry and grape plants. Demethylation-inhibitor fungicides (DMIs) are potential alternatives to benzimidazole fungicides for the management of anthracnose in strawberry and grape plants in China. However, no information regarding the baseline sensitivity of C. gloeosporioides complex to DMIs is available. In this study, a total of 216 isolates of C. gloeosporioides including 113 from strawberries and 103 from grapes, were tested for their baseline sensitivities to prochloraz and tebuconazole. The sensitivities of the total combined population (n = 216) of the strawberry and grape isolates collected from six different locations showed no geographical variation. The frequency distribution of the EC50 values for the 216 isolates was a unimodal curve. The mean EC50 value of 0.053 ± 0.01 mg/l (ranging from 0.016 to 0.19 mg/l), and 0.62 ± 0.11 mg/l (0.27 to 3.75 mg/l), could be utilized as the baseline for monitoring the shift of sensitivity to prochloraz and tebuconazole, respectively. Some geographical variation was detected among the grape subpopulation (n = 103), which showed significantly lower sensitivity to both fungicides than the strawberry subpopulation (n = 113). Prochloraz and tebuconazole had a significantly lower risk of resistance development than that of kresoxim-methyl, which is a Qo inhibitor. This was indicated by the fewer mutants obtained through UV mutagenesis, the lower resistant factor and the decreased sporulation and pathogenicity ability of the obtained mutants. All of the kresoxim-methyl resistant mutants became resistant to pyraclostrobin, but not to the boscalid or DMIs. Interestingly, however, a positive cross-resistance was observed between tebuconazole and difenoconazole, but not between tebuconazole and prochloraz.
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References
Agostini JP, Timmer LW (1992) Selective isolation procedures for differentiation of two strains of Colletotrichum gloeosporioides from citrus. Plant Dis 76:1176–1178
Akhter M, Alam S, Islam M, Lee MW (2009) Identification of the fungal pathogen that causes strawberry anthracnose in Bangladesh and evaluation of in vitro fungicide activity. Mycobiology 37:77–81
Bartlett DW, Clough JM, Godwin JR, Hall AA, Hamer M, Parr-Dobrzanski B (2002) The strobilurin fungicides. Pest Manag Sci 58:649–662
Brent KJ, Hollomon DW (1998) Fungicide resistance: the assessment of risk. FRAC Monograph 2, GCPF, Brussels (Now CropLife International), 48pp. Available on line at www.frac.info
Cannon PF, Buddie AG, Bridge PD (2008) The typification of Colletotrichum gloeosporioides. Mycotaxon 104:189–204
Cannon PF, Damm U, Johnston PR, Weir BS (2012) Colletotrichum–current status and future directions. Stud Mycol 73:181–213
Damm U, Baroncelli R, Cai L, Kubo Y, O’Connell R, Weir B, Cannon PF (2010) Colletotrichum: species, ecology and interactions. IMA Fungus Glob Mycol J 1:161–165
Freeman S, Katan T, Shabi E (1998) Characterization of Colletotrichum species responsible for anthracnose disease of various fruits. Plant Dis 82:596–604
Han GX, Li Q, Sun FZ, Li HY (2009) Identification of pathogen caused strawberry anthracnose and their resistance to carbendazim and diethofencarb. Zhejiang Agric Sci 6:1169–1172
Hsiang T, Yang L, Barton W (1997) Baseline sensitivity and cross-resistance to demethylation-inhibiting fungicides in Ontario isolates of Sclerotinia homoeocarpa. Eur J Plant Pathol 103:409–416
Hulvey J, James TP, Sang H, Berg A, Jung G (2012) Overexpression of ShCYP51B and ShatrD in Sclerotinia homoeocarpa isolates exhibiting practical field resistance to a demethylation inhibitor fungicide. Appl Environ Microbiol 78:6674–6682
Indu SS, Shubhangi PN, Dinesh SS, Anuradha U, Sawant SD (2012) Emergence of Colletotrichum gloeosporioides sensu lato as the dominant pathogen of anthracnose disease of grapes in India as evidenced by cultural, morphological and molecular data. Australas Plant Pathol 41:493–504
Jones S, Pethybridge S, Hay F, Groom T, Wilson C (2007) Baseline sensitivity of Australian Phoma ligulicola isolates from pyrethrum to azoxystrobin and difenoconazole. J Phytopathol 155:377–380
Ma Z, Michailides TJ (2005) Advances in understanding molecular mechanisms of fungicide resistance and molecular diagnosis of resistant genotypes of phytopathogenic fungi. Crop Prot 24:853–863
Malandrakis A, Koukiasas N, Veloukas T, Karaoglanidis G, Markoglou A (2013) Baseline sensitivity of Monilinia laxa from Greece to fenhexamid and analysis of fenhexamid-resistant mutants. Crop Prot 46:13–17
Mavroeidi VI, Shaw MW (2005) Sensitivity distributions and cross-resistance patterns of Mycosphaerella graminicola to fluquinconazole, prochloraz and azoxystrobin over a period of 9 years. Crop Prot 24:259–266
McManus PS, Best VM, Voland RP, Leininger BL (1999) Sensitivity of Monilinia oxycocci to fenbuconazole and propiconazole in vitro and control of cranberry cottonball in the field. Plant Dis 83:445–450
O’Connell RJ, Perfect S, Hughes B, Carzaniga R, Bailey JA, Green J (2000) Dissecting the cell biology of Colletotrichum infection processes. Host specificity, pathology, and host–pathogen interaction of Colletotrichum. American Phytopathology Society Press, St Paul, pp 57–77
Robbertse B, Holz G, Crous PW (1996) Sensitivity of South African Ramulispora herpotrichoides isolates to carbendazim and ergosterol biosynthesis inhibitors. Plant Pathol 45:270–275
Russell PE (2004) Sensitivity baselines in fungicide resistance research and management. In: FRAC Monograph, vol. 3. CropLife International, Brussels. Available on line at: www.frac.info
Weir BS, Johnston PR, Damm U (2012) The Colletotrichum gloeosporioides species complex. Stud Mycol 73:115–180
Zhang CQ, Yuan SK, Sun HY, Qi ZQ, Zhou MG, Zhu GN (2007) Sensitivity of Botrytis cinerea to boscalid. Plant Pathol 56:646–653
Zhang CQ, Liu YH, Zhu GN (2010) Detection and characterization of benzimidazole resistance of Botrytis cinerea in greenhouse vegetables. Eur J Plant Pathol 126:509–515
Zhang CQ, Liu YH, Wu HM, Xu BC, Sun PL, Xu ZH (2012) Baseline sensitivity of Pestalotiopsis microspora, which causes black spot disease on Chinese hickory (Carya cathayensis), to pyraclostrobin. Crop Prot 42:256–259
Zhao MZ, Wang J, Wang ZW, Qian YM, Wu WM (2012) Strawberry production and trade in the world. Fruit Grow Friend 6:38
Acknowledgments
This research was partially supported by the Special Fund for Agro-Scientific Research in the Public Interest (No. 201303023) and the Project of Six-party of Agriculture, Rural areas and Peasantry in Zhejiang Province.
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X. F Xu and T. Lin contributed equally to this work.
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Xu, X.F., Lin, T., Yuan, S.K. et al. Characterization of baseline sensitivity and resistance risk of Colletotrichum gloeosporioides complex isolates from strawberry and grape to two demethylation-inhibitor fungicides, prochloraz and tebuconazole. Australasian Plant Pathol. 43, 605–613 (2014). https://doi.org/10.1007/s13313-014-0321-8
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DOI: https://doi.org/10.1007/s13313-014-0321-8