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Upregulation of plasmacytoid dendritic cells in glioma

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Tumor Biology

Abstract

The immune system fails to eradicate established tumors partly due to the induction of immune tolerance within tumor microenvironment. Plasmacytoid dendritic cells (pDCs) play critical roles in regulating immune system. In this study, we investigated pDC in the peripheral blood of glioma. CD4 + CD123 + BDCA2+ pDCs were tested from peripheral blood mononuclear cells in 40 glioma patients and 40 healthy controls by flow cytometry. The results revealed that proportion of pDCs was significantly increased in cases than in controls (0.52 ± 0.07 versus 0.21 ± 0.02 %, p < 0.001), whereas myeloid dendritic cells (mDCs) did not present any obvious difference between patients and healthy donors (0.25 ± 0.04 versus 0.18 ± 0.02 %, p = 0.217). We further studied pDCs in glioma patients with different clinical stages. Data showed that cases with smoking history had elevated level of pDCs than those non-smoker patients (0.91 ± 0.16 versus 0.48 ± 0.06 %, p = 0.004). Interestingly, we observed that patients with aphasia presented significantly elevated pDCs than those without aphasia (0.93 ± 0.12 versus 0.41 ± 0.07 %, p < 0.001). These data suggested that pDCs may be closely involved in the pathogenesis of glioma and may play roles in certain symptoms of the disease.

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References

  1. Han S, Feng S, Yuan G, Dong T, Gao D, et al. Lysyl oxidase genetic variants and the prognosis of glioma. APMIS. 2014;122:200–5.

    Article  CAS  PubMed  Google Scholar 

  2. Bashir T, Cloninger C, Artinian N, Anderson L, Bernath A, et al. Conditional astroglial Rictor overexpression induces malignant glioma in mice. PLoS One. 2012;7:e47741.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Robinson JP, VanBrocklin MW, Guilbeault AR, Signorelli DL, Brandner S, et al. Activated BRAF induces gliomas in mice when combined with Ink4a/Arf loss or Akt activation. Oncogene. 2010;29:335–44.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Zen M, Canova M, Campana C, Bettio S, Nalotto L, et al. The kaleidoscope of glucocorticoid effects on immune system. Autoimmun Rev. 2011;10:305–10.

    Article  CAS  PubMed  Google Scholar 

  5. Zhang HG, Grizzle WE. Exosomes and cancer: a newly described pathway of immune suppression. Clin Cancer Res. 2011;17:959–64.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Yu XH, Jiang N, Zheng XL, Cayabyab FS, Tang ZB, et al. Interleukin-17A in lipid metabolism and atherosclerosis. Clin Chim Acta. 2014;431C:33–9.

    Article  Google Scholar 

  7. Yuan X, Wei Q, Komaki R, Liu Z, Yang J, et al. Polymorphisms predict distant metastasis-free survival in patients with inoperable non-small-cell lung cancer after definitive radiotherapy. PLoS One. 2013;8:e65659.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Yang S, Wang C, Zhou Y, Sun G, Zhu D, et al. Cytotoxic T-lymphocyte antigen-4 polymorphisms and susceptibility to Ewing’s sarcoma. Genet Test Mol Biomark. 2012;16:1236–40.

    Article  CAS  Google Scholar 

  9. Yu H, Zou X, Peng L, Wang Y, Zhang C, et al. Effect of soluble inducible costimulator level and its polymorphisms on age-related macular degeneration. DNA Cell Biol. 2013;32:717–21.

    Article  PubMed Central  PubMed  Google Scholar 

  10. **ang H, Zhao W, Sun Y, Qian W, **ng J, et al. CD86 gene variants and susceptibility to pancreatic cancer. J Cancer Res Clin Oncol. 2012;138:2061–7.

    Article  CAS  PubMed  Google Scholar 

  11. Xu P, Chen YJ, Chen H, Zhu XY, Song HF, et al. The expression of programmed death-1 in circulating CD4+ and CD8+ T cells during hepatitis B virus infection progression and its correlation with clinical baseline characteristics. Gut Liver. 2014;8:186–95.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Yamahira A, Narita M, Nakamura T, Watanabe N, Kaji M, et al. Generation of antigen-specific cytotoxic T lymphocytes using a leukemic plasmacytoid dendritic cell line as antigen presenting cells. Leuk Res. 2011;35:793–9.

    Article  CAS  PubMed  Google Scholar 

  13. Wei L, Liu B, Tuo J, Shen D, Chen P, et al. Hypomethylation of the IL17RC promoter associates with age-related macular degeneration. Cell Rep. 2012;2:1151–8.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Wendland M, Willenzon S, Kocks J, Davalos-Misslitz AC, Hammerschmidt SI, et al. Lymph node T cell homeostasis relies on steady state homing of dendritic cells. Immunity. 2011;35:945–57.

    Article  CAS  PubMed  Google Scholar 

  15. Wergeland I, Assmus J, Dyrhol-Riise AM. T regulatory cells and immune activation in Mycobacterium tuberculosis infection and the effect of preventive therapy. Scand J Immunol. 2011;73:234–42.

    Article  CAS  PubMed  Google Scholar 

  16. Adachi K, Davis MM. T-cell receptor ligation induces distinct signaling pathways in naive vs. antigen-experienced T cells. Proc Natl Acad Sci U S A. 2011;108:1549–54.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Ait-Oufella H, Sage AP, Mallat Z, Tedgui A. Adaptive (T and B cells) immunity and control by dendritic cells in atherosclerosis. Circ Res. 2014;114:1640–60.

    Article  CAS  PubMed  Google Scholar 

  18. Anguille S, Smits EL, Lion E, van Tendeloo VF, Berneman ZN. Clinical use of dendritic cells for cancer therapy. Lancet Oncol. 2014;15:e257–67.

    Article  CAS  PubMed  Google Scholar 

  19. Dietel B, Muench R, Kuehn C, Kerek F, Steinkasserer A, et al. MCS-18, a natural product isolated from Helleborus purpurascens, inhibits maturation of dendritic cells in ApoE-deficient mice and prevents early atherosclerosis progression. Atherosclerosis. 2014;235:263–72.

    Article  CAS  PubMed  Google Scholar 

  20. Dubrot J, Duraes FV, Potin L, Capotosti F, Brighouse D, et al. Lymph node stromal cells acquire peptide-MHCII complexes from dendritic cells and induce antigen-specific CD4+ T cell tolerance. J Exp Med. 2014;211:1153–66.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Shi W, Li X, Porter JL, Ostrodi DH, Yang B, et al. Level of plasmacytoid dendritic cells is increased in non-small cell lung carcinoma. Tumour Biol. 2014;35:2247–52.

    Article  CAS  PubMed  Google Scholar 

  22. Green TL, Santos MF, Ejaeidi AA, Craft BS, Lewis RE, et al. Toll-like receptor (TLR) expression of immune system cells from metastatic breast cancer patients with circulating tumor cells. Exp Mol Pathol. 2014;97:44–8.

    Article  CAS  PubMed  Google Scholar 

  23. Tel J, Hato SV, Torensma R, Buschow SI, Figdor CG, et al. The chemotherapeutic drug oxaliplatin differentially affects blood DC function dependent on environmental cues. Cancer Immunol Immunother. 2012;61:1101–11.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. Troegeler A, Lastrucci C, Duval C, Tanne A, Cougoule C, et al. (2014) An efficient siRNA-mediated gene silencing in primary human monocytes, dendritic cells and macrophages. Immunol Cell Biol.

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Authors and Affiliations

  1. Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, 130033, China

    Rui Wang

  2. Department of Vascular and Endocrine Surgery, ****g Hospital, The Fourth Military Medical University, **’an, Shaanxi Province, 710032, China

    Ju-Liang Zhang

  3. Department of Neurosurgery, China-Japan Union Hospital of Jilin University, 126 **antai Street, Changchun, Jilin Province, 130033, China

    Bo Wei, Yu Tian, Zhao-Hui Li & Chao Du

  4. Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, 130033, China

    Le Wang

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  1. Rui Wang
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  2. Ju-Liang Zhang
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  3. Bo Wei
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  4. Yu Tian
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  5. Zhao-Hui Li
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  6. Le Wang
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  7. Chao Du
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Correspondence to Chao Du.

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Rui Wang and Ju-Liang Zhang contributed equally to the work.

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Wang, R., Zhang, JL., Wei, B. et al. Upregulation of plasmacytoid dendritic cells in glioma. Tumor Biol. 35, 9661–9666 (2014). https://doi.org/10.1007/s13277-014-2211-7

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  • DOI: https://doi.org/10.1007/s13277-014-2211-7

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