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Myostatin-2 isolation and spatiotemporal expression comparison between myostatin-1 and -2 in Larimichthys crocea

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Abstract

The 3,110-bp myostatin-2 was obtained from a marine teleost, Larimichthys crocea (L. crocea), by PCR amplication coupled with 5′- and 3′-rapid amplification of cDNA ends. Myostatin-2 contains a 71-bp 5′-untranslated region, a 328-bp exon I, an 895-bp intron I, a 371-bp exon II, a 382-bp intron II, a 381-bp exon III, and a 682 bp 3′-untranslated region, and it encodes a protein of 359 amino acid residues. The deduced amino acid sequence has typical characteristics of TGF-β family members with nine conserved cysteine residues and a RXXR proteolytic processing site within the conserved C-terminal portion, and more than 90 % similarity with other fish myostatin-2 proteins. Quantitative assessment revealed that levels of myostatin-1 transcripts fluctuated markedly, whereas myostatin-2 expression remained very low during most stages of embryonic development. In addition, both myostatin-1 and -2 transcripts were detected in all 10 examined tissues of juvenile and adult individuals, but they exhibited different expression patterns and responses to fasting. Expression of myostatin-1 was highest in skeletal muscle, which was higher than myostatin-2 by 2–3 orders of magnitude. Myostatin-2 transcripts were greatest in brain. Myostatin-1 expression increased significantly after 3 days of fasting, whereas myostatin-2 expression was unaffected. Our results imply that myostatin-1 and -2 may have different roles in skeletal muscle growth and development of L. crocea.

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Acknowledgments

The project was supported by National Natural Science Foundation of China (Grant Nos. 30871916 and 31172398), and Agricultural Project of Science and Technology Department of Zhejiang Province (Grant No. 2011C22087) and of Ningbo Municipality of China (Grant Nos. 2011C10001 and 2011B82018) to Xue. This article is sponsored by K. C. Wong Magna Fund in Ningbo University. Authors thank Mr. Mehary Aynealem Gebremariam for his proofreading the manuscript.

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. College of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, 315211, Zhejiang, People’s Republic of China

    Zhifei Liu, Liangyi Xue, Sheng Sun, Zhen Xu & Hong Yu

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  1. Zhifei Liu
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  2. Liangyi Xue
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Correspondence to Liangyi Xue.

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Liu, Z., Xue, L., Sun, S. et al. Myostatin-2 isolation and spatiotemporal expression comparison between myostatin-1 and -2 in Larimichthys crocea . Genes Genom 36, 599–609 (2014). https://doi.org/10.1007/s13258-014-0196-7

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