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DNA markers in analysis of genetic diversity of Curcuma longa L. from Meghalaya

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Abstract

DNA markers viz. DAMD, ISJ and SRAP were used to characterize intra-specific genetic diversity of Curcuma longa (turmeric) collections from Meghalaya to evince levels of molecular variability and genetic diversity among the genotypes. Comparison of the degree of polymorphism and efficacy of the different molecular markers showed a high degree of polymorphism (> 93%) and high number of effective alleles (> 1.9) reflecting a relatively high genetic diversity among turmeric collections. UPGMA clustering pattern of DAMD, ISJ and SRAP revealed a mixed clustering pattern forming three major clusters. Overall, DNA-based markers used in the study were found to be efficient and highly informative. However, ISJ markers are more promising and can be considered as important tools for a better understanding of genetic relationships among the accessions and/or germplasm, and in selecting unique gene-pool for further biotechnological innovation and exploitation of C. longa germplasm.

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Acknowledgements

The authors are thankful to the Head of the Department for providing necessary facilities. The authors are also grateful to the Department of Biotechnology, Government of India for financial assistance (Ref. No. DBTJRF/2010-11/582 dated 27th January, 2011). Sincere thanks are also due to the members of Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong for their constant help and encouragement.

Funding

Department of Biotechnology, Government of India (Ref. No. DBTJRF/2010-11/582 dated 27th January, 2011).

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Authors and Affiliations

  1. Department of Biotechnology, St. Anthony’s College, Shillong, Meghalaya, India

    Judith Mary Lamo

  2. Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya, India

    Satyawada Rama Rao

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SRR conceived and supervised the study; JML collected samples and performed experimental and data analysis, both the authors wrote and finalized the manuscript.

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Correspondence to Judith Mary Lamo.

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Lamo, J.M., Rao, S.R. DNA markers in analysis of genetic diversity of Curcuma longa L. from Meghalaya. Nucleus 66, 127–136 (2023). https://doi.org/10.1007/s13237-023-00424-8

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