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Comparative study on intestinal bacterial communities of Boleophthalmus pectinirostris and Periophthalmus magnuspinnatus with different sexes and feeding strategies

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Abstract

Intertidal mudflats are unique, highly productive ecosystems. Boleophthalmus pectinirostris and Periophthalmus magnuspinnatus are common fish species that are distributed in the intertidal mudflats of the Yangtze Estuary in China. They perform important ecological functions and have different feeding strategies. Herein, we studied the intestinal microbial diversity and structure of wild B. pectinirostris and P. magnuspinnatus with different sexes and feeding strategies during their breeding season. Gut samples of B. pectinirostris and P. magnuspinnatus individuals (female:male ratio = 1:1) were collected and subjected to high-throughput DNA sequencing. The results showed Proteobacteria was the most dominant phylum in all the four sample groups: 73.5% in the males and 52.6% in the females of B. pectinirostris and 40.2% in the males and 40.9% in the females of P. magnuspinnatus. Aeromonas, Shewanella, Halomonas, and Acinetobacter of the phylum Proteobacteria were dominant genera in all the sample groups and accounted for 62.13% of the ten dominant genera. The diversity of the intestinal microflora in the omnivorous P. magnuspinnatus was significantly higher (P < 0.05) than that in the herbivorous B. pectinirostris. Beta diversity, including PCoA and UPGMA of unweighted UniFrac distances, showed that B. pectinirostris samples were clustered together, and P. magnuspinnatus samples were clustered together, implying the effect of the feeding habits on the microbial community structure is more considerable than that of sex.

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Funding

This work was financially supported by the NSFC (31600334).

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Author notes

  1. Key Laboratory of Fisheries Ecology of the Yangtze Estuary, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences and College of Fisheries and Life Science, Shanghai Ocean University contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Fisheries Ecology of the Yangtze Estuary, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Yangpu District, Shanghai, 200090, People’s Republic of China

    Rongrong Ma, Sikai Wang, Feng Zhao, Chao Xu, Yan Ji, Chao Song, Yu Wang & ** Zhuang

  2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China

    Rongrong Ma & Yan Ji

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  1. Rongrong Ma
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Correspondence to ** Zhuang.

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The study was reviewed and approved by the Ethics Committee of East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences.

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Ma, R., Wang, S., Zhao, F. et al. Comparative study on intestinal bacterial communities of Boleophthalmus pectinirostris and Periophthalmus magnuspinnatus with different sexes and feeding strategies. Ann Microbiol 68, 123–133 (2018). https://doi.org/10.1007/s13213-018-1324-4

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