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Immunogenicity Monitoring Cell Chip Incorporating Finger-Actuated Microfluidic and Colorimetric Paper-Based Analytical Functions

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Abstract

Recently, with the development of microfluidic chips, attempts to integrate cell culture and biomedical material testing functions into a single chip have increased to supplement experimental animal models. Among the evaluations of biomaterials, immunogenicity testing, a current priority, is attracting attention. In this study, we developed a simple and easy-to-handle immunogenicity-testing cell chip to evaluate the immunogenicity of biomaterials. On this chip, macrophages were introduced as immunogenicity indicators, and a micro-paper-based analytical device (µPAD) was used for optical analysis. Macrophages are present in all parts of the body and mediate immune reactions against body implants or bio-derived substances, resulting in the production of hydrogen peroxide. In the cell chamber of the developed cell chip, macrophages grow and react to immunogenic materials. Activated macrophages secrete hydrogen peroxide, which is then transferred to the PAD with single-finger actuation. The hydrogen peroxide molecules reaching the PAD detection zone react with the colorimetric detection substrate, resulting in a color that corresponds to the hydrogen peroxide concentration. With the developed testing chip, the immunogenicity of biomaterials can be determined before administration.

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Acknowledgements

This work was supported by the Creative Materials Discovery Program (NRF-2019M3D1A1078943) and research grants (NRF-2019R1A6A1A11051471, NRF-2021R1A2C3004180) funded by the National Research Foundation of Korea. H.C.Y also acknowledges the support from the Commercialization Promotion Agency for R & D Outcomes grant funded by the Korean government (2021N100).

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  1. Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea

    Kyung Won Lee, Eun Kyeong Yang, Yujeong Oh, Eunhye Park, Kwan Young Jeong & Hyun C. Yoon

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Correspondence to Hyun C. Yoon.

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Lee, K.W., Yang, E.K., Oh, Y. et al. Immunogenicity Monitoring Cell Chip Incorporating Finger-Actuated Microfluidic and Colorimetric Paper-Based Analytical Functions. BioChip J 17, 329–339 (2023). https://doi.org/10.1007/s13206-023-00111-5

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