Abstract
Jatropha, a popular biodiesel crop, suffers severe losses due to Jatropha leaf curl Gujarat virus (JLCuGV) infection in Gujarat (India). Metabolite profiling can help to understand the plant's innate immune response to geminivirus infection. Our study aims to compare metabolic profiles of an infected and healthy plant to unravel the changes in biochemical pathways on geminivirus infection in Jatropha. Gas chromatography–mass spectrometry (GC–MS) analysis was performed in healthy and infected tissue of Jatropha field plants which were identified to be infected with geminivirus. GC–MS analysis revealed that the metabolites like sugars, polyols, carboxylic acids, fatty acids, polyphenols, and amino acids were regulated on JLCuGV infection. The sugars (glucose, sucrose, and fructose) increased, while carboxylic acids (malic acid, citric acid and quinic acid) and polyols (galactinol, butanetriol, triethylene glycol, myo-inositol, erythritol) decreased remarkably in infected Jatropha tissue. All these metabolic variations indicated that sugar metabolism and tricarboxylic acid (TCA) cycle pathways are regulated as a defense response and a disease development response to geminivirus infection in Jatropha.
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Acknowledgements
CSIR-CSMCRI Communication No.-PRIS 135/2020. The authors are thankful to the Department of Science and Technology (DST) and the Council of Scientific and Industrial Research (CSIR), New Delhi, India. PM is supported by the DST-INSPIRE SRF fellowship. PA acknowledges the financial support from the DST-WOS-A scheme.
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PM performed major experiments. PA helped in designing and executing experiments. PA and PKA conceived and coordinated the experiments. All authors have contributed to MS writing and approved the final manuscript.
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More, P., Agarwal, P. & Agarwal, P.K. The Jatropha leaf curl Gujarat virus on infection in Jatropha regulates the sugar and tricarboxylic acid cycle metabolic pathways. 3 Biotech 12, 275 (2022). https://doi.org/10.1007/s13205-022-03306-z
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DOI: https://doi.org/10.1007/s13205-022-03306-z