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Whole genome characterization of wisteria vein mosaic virus from Iran and its relationship to other members of bean common mosaic virus group

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Abstract

To date, the complete genome of two wisteria vein mosaic virus (WVMV) has been sequenced worldwide. Here, the genomic sequence of WVMV isolated from Wisteria sinensis in Iran was determined for the first time, using deep RNA sequencing and RT-PCR followed by Sanger sequencing. The sequence was 9694 nucleotides in length; excluding the 3’-poly(A) tail and contained a single open reading frame of 9279 nucleotides encoding a large polyprotein of 3092 amino acids and predicted molecular weight of 35,368 KDa. The genome contained nine putative proteolytic cleavage sites and motifs conserved in homologous proteins of other potyviruses. Sequence analysis suggested that WVMV-Ir sequence shared 76.37–86.01% nucleotide (nt) identity and 82.45–91.91% amino acid (aa) identity with two other isolates (Bei**g and JEBU-p) available in the GenBank, the highest with the Chinese isolate Bei**g (86.01% nt identity, 91.91% aa identity). Sequence identities over most of the genome were within the range 80–86% and 85–95% at the nt and aa levels, respectively; however, high variability was observed in the 5’-UTR (51.62%), P1 (62.03% nt identity, 50.78% aa identity) and P3 (79.82%nt identity, 78.67% aa identity) regions, suggesting that Ir, Bei**g, and JEBU-p are three different strains. These variabilities may be due to different mutation phenomena of a common ancestor virus or mutations caused by different selection pressures in different agro-ecological regions. The results of the phylogenetic analysis indicated that WVMV was most closely related to soybean mosaic virus and watermelon mosaic virus and less closely related to the zantedeschia mild mosaic virus and dasheen mosaic virus. In the greenhouse, WVMV-Ir caused severe symptoms in Phaseolus vulgaris, Vicia faba, W. sinensis, Chenopodium quinoa, C. amaranticolor, and Nicotiana benthamiana.

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Author notes

  1. Mohanad S. Al-Jaberi and Zohreh Moradi authors contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Mohanad S. Al-Jaberi, Mohsen Mehrvar, Hayder R. Al-Inizi & Mohammad Zakiaghl

  2. Department of Plant Pathology, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. BOX: 578, Sari, Iran

    Zohreh Moradi

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  1. Mohanad S. Al-Jaberi
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  2. Zohreh Moradi
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  3. Mohsen Mehrvar
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  4. Hayder R. Al-Inizi
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  5. Mohammad Zakiaghl
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Contributions

MM conceived and designed the experiments. MSAJ did the experimental works. ZM and HRAI helped to conduct some laboratory experiments. ZM prepared the manuscript, performed data analysis, and interpretation. ZM, MM, MZ and MSAJ contributed to the interpretation and critical revision of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Mohsen Mehrvar.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Al-Jaberi, M.S., Moradi, Z., Mehrvar, M. et al. Whole genome characterization of wisteria vein mosaic virus from Iran and its relationship to other members of bean common mosaic virus group. 3 Biotech 11, 407 (2021). https://doi.org/10.1007/s13205-021-02957-8

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