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Optimization of growth conditions for enhancing the production of microbial laccase and its application in treating antibiotic contamination in wastewater

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Abstract

In this work, seven indigenous macrofungal isolates were selected to screen for their laccase production capability. Among them, isolates viz., Pleurotus eryngii, Pleurotus florida, Pleurotus sajor caju and Gandoderma lucidum were found to exhibit high laccase activity in the preliminary studies and were thus selected for the optimization studies with an aim to enhance laccase production. The pH optimization studies were carried out between pH range of 4–6. The laccase activity and biomass were found to be optimum at pH 4, 4.5, 4.5 and 5 for P. eryngii, P. florida, P. sajor caju and G. lucidum, respectively. Optimization studies with chemical inducers namely, tannic acid, 2,6 dimethoxyphenol and copper sulphate at three different concentration levels were conducted and tannic acid at 2 mM concentration was found to increase the laccase activity to about 45% followed by 2,6 dimethoxyphenol (2 mM) with an increase of about 43% and copper sulphate (0.1 mM) showing 21% increase in the yield. Biodegradation studies utilizing laccase isolated from P. eryngii, P. florida and P. sajor caju was carried out for a commonly detected fluoroquinolone antibiotic, levofloxacin, in water and pharmaceutical wastewater. The results indicated that the degradation efficiency of levofloxacin using laccase isolated from P. eryngii (88.9%) was comparable to commercial laccase (89%). When the cost economics of using crude laccase was evaluated against commercial laccase it was evident that the total cost of the treatment could be reduced by 71.7% if commercial grade laccase was replaced by crude enzyme extracted from indigenous macrofungi such Pleurotus eryngii, Pleurotus florida, and Pleurotus sajor caju indicating a promising and cost-effective alternative for wastewater treatment.

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Acknowledgements

We thank The Director, Sustainable Agriculture Division, TERI Deakin Nanobiotechnology Centre, The Energy and Resources Institute, New Delhi for providing necessary facilities to carry out the research work. Special thanks are also extended to the supervisory panel members of TERI Deakin Nanobiotechnology Centre, The Energy and Resources Institute, New Delhi (Dr Pawan Kaur) and School of Life and Environmental Sciences, Burwood Campus, Deakin University Australia (Dr Damien Callahan, Dr Xavier Conlan and Dr Fred Pfeffer).

Funding

This work, which is part of Purvi Mathur's doctoral thesis work, is jointly supported by the TERI Deakin Nanobiotechnology Centre, The Energy and Resources Institute, New Delhi and School of Life and Environmental Sciences, Burwood Campus, Deakin University Australia.

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Authors and Affiliations

  1. TERI-Deakin NanoBiotechnology Centre, Sustainable Agriculture Division, The Energy and Resources Institute, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi, 110003, India

    Purvi Mathur & Doyeli Sanyal

  2. School of Life and Environmental Sciences, Deakin University, Burwood Campus, 221 Burwood Highway, Burwood, Melbourne, VIC, 3125, Australia

    Purvi Mathur

  3. Centre for Mycorrhiza Research, The Energy and Resources Institute, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi, 110003, India

    Pannalal Dey

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  1. Purvi Mathur
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Correspondence to Doyeli Sanyal.

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Mathur, P., Sanyal, D. & Dey, P. Optimization of growth conditions for enhancing the production of microbial laccase and its application in treating antibiotic contamination in wastewater. 3 Biotech 11, 81 (2021). https://doi.org/10.1007/s13205-020-02627-1

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