Abstract
The present study was designed to explore the role of microRNA-197-3p in regulating the epithelial–mesenchymal cellular transition in ovarian cancer. The results showed that miR-197 to be significantly (P < 0.05) downregulated in human ovarian cancer tissues and cell lines. Overexpression of miR-197 significantly (P < 0.05) reduced the proliferation of OVACAR-3 cancer cells. Additionally, the colony formation of the OVACAR-3 cells was inhibited by 59% relative to control. The migration and invasion of the OVACAR-3 cells was inhibited by 64% and 72%, respectively, upon miR-197 overexpression. Western blot analysis showed miR-197 was found to upregulate the expression of E-cadherin, while the expression of N-cadherin, vimentin, and snail proteins was found to decrease significantly (P < 0.05). TargetScan analysis together with dual luciferase assay revealed that miR-197 exerts its effects by targeting ABCA7 in ovarian cancer. ABCA7 was significantly (P < 0.05) overexpressed in ovarian cancer tissues and cell lines. However, silencing of ABCA7 resulted in significant inhibition of cell proliferation, migration, and invasion. Nonetheless, overexpression of ABCA7 could abolish the tumor-suppressive effects of miR-197 on the OVACAR-3 cells. Taken together, miR-197 acts a tumor-suppressive in ovarian cancer and points towards its therapeutic implications in the treatment of ovarian cancer.
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Acknowledgements
The authors acknowledge the experimental assistance from The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, China
Funding
This research did not obtain any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
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Authors and Affiliations
Contributions
Conceptualization: WX and XF; methodology: WX. CS, YP, and XF; formal analysis and investigation: WX, CS, and YP; writing—original draft preparation: XF, LQ, and WX; writing—review and editing critically for important intellectual content: XF and LQ; supervision: XF.
