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Optimization of parameters for the production of biodiesel from rubber seed oil using onsite lipase by response surface methodology

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Abstract

In the present study, we demonstrate the properties of rubber seed oil (RSO) and its potential application for producing biodiesel by lipase-catalyzed transesterification. Rubber seed contains 39.45% of oil on a dry weight basis. Lipase was secreted from novel bacterial isolate of Pseudomonas aeruginosa strain BUP2. Response Surface Methodology (RSM) cum Box–Behnken Design (BBD) was employed to optimize the combined effect of different independent parameters namely oil–methanol ratio, enzyme unit, reaction temperature and reaction time. Biodiesel yield of 99.52% was obtained in the validation experiments at the optimal level of lipase (750 U), methanol ratio (1:10), temperature (45 °C) and time (4 h). The fuel properties of biodiesel obtained under the validation condition met the specifications as mentioned in ASTM D6751 and EN 14214 standards. Biodiesel aliquots were characterized using thin-layer chromatography (TLC), gas chromatography (GC), fourier transform infra-red spectroscopy (FTIR) and elemental analysis. The present study demonstrates an important application of a potential substitute for fossil fuel from raw feedstocks with high economic value.

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Acknowledgements

The financial assistance (Grant no. 1417/2014/KSCSTE) of Kerala State Council for Science, Technology and Environment (KSCSTE), Government of Kerala is gratefully acknowledged. The authors also express their gratitude to Prof. Sailas Benjamin (Ex-Professor, University of Calicut) for his immense support, help and guidance during the execution of the work.

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Authors and Affiliations

  1. Department of Botany, School of Biosciences, University of Calicut, Kerala, 673635, India

    Abdul Faisal Panichikkal, Unni Kizhakkepowathial Nair & Mohanan Kulangara Valappil

  2. Department of Biotechnology, Cochin University of Science and Technology, Cochin, Kerala, 682022, India

    Priji Prakasan

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  1. Abdul Faisal Panichikkal
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Correspondence to Abdul Faisal Panichikkal.

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13205_2018_1477_MOESM1_ESM.docx

Fig. S 1 Diagrammatic representation of reaction setup. Fig. S2 Gas chromatogram of A) FAMEs obtained from rubber seed oil; B) Rubber seed oil. (DOCX 463 KB)

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Panichikkal, A.F., Prakasan, P., Kizhakkepowathial Nair, U. et al. Optimization of parameters for the production of biodiesel from rubber seed oil using onsite lipase by response surface methodology. 3 Biotech 8, 459 (2018). https://doi.org/10.1007/s13205-018-1477-7

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