Abstract
Environmental stresses, particularly salt stress are one of the most restricting factors of crop performance. The effects of salinity stress levels S0 (EC 1 mmho/cm), S1 (EC 10 mmho/cm), S2 (EC 15 mmho/cm), and S3 (EC 20 mmho/cm) on morphological, physiological, and biochemical parameters in two winter wheat cultivars (Lalmi-4 and Kabul-013) were investigated in this study. The results indicated that salinity negatively affects plant height, tiller number, plant biomass, and days to heading of both cultivars, with more pronounced effects on Kabul-013. The physiological and biochemical reasons for the reduction could be attributed to some extent to higher cellular membrane damage, an increased rate of lipid peroxidation, and maintaining osmoregulation in Kabul-013. Moderate and high salinities increased the leaf electrolyte leakage (EL), malondialdehyde (MDA), and leaf proline contents (LPC) in Kabul-013 as compared to Lalmi-4. With rising salt concentrations, yield and its components declined in both cultivars. However, the Lalmi-4 cultivar reveals more tolerance to salinity stress compared to the Kabul-013 cultivar, possibly by better growth performance, assembling fewer MDA and proline contents, and a lower value of leaf electrolyte leakage as well as producing more grain yield. According to the findings of this study, salt stress reduces overall wheat crop performance by modifying its physiological and biochemical pathways. Utilizing the discovery of the wheat whole genome sequence, more study is required to pinpoint the genes, metabolites, and pathways responsible for the many processes of salt tolerance in wheat. In view of the present expansion of biotechnological technology, multidisciplinary approaches to the development of salt-tolerant wheat cultivars are highly encouraged.
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References
Ahmed HGM-D, Zeng Y, Raza H, Muhammad D, Iqbal M, Uzair M, Khan MA, Iqbal R, El Sabagh A (2022) Characterization of wheat (Triticum aestivum L.) accessions using morpho-physiological traits under varying levels of salinity stress at seedling stage. Front Plant Sci 13:953670
Akbarimoghaddam H, Galavi M, Ghanbari A, Panjehkeh N (2011) Salinity effects on seed germination and seedling growth of bread wheat cultivars. Trakia J Sci 9:43–50
Arzani A, Ashraf M (2017) Cultivated ancient wheats (Triticum spp.): a potential source of health-beneficial food products. Compr Rev Food Sci Food Saf 16:477–488
Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216
Ashraf M, Ali Q (2008) Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L.). Environ Exp Bot 63:266–273
Bajji M, Bertin P, Lutts S, Kinet JM (2004) Evaluation of drought resistance-related traits in durum wheat somaclonal lines selected in vitro. Aust J Exp Agric 44:27–35
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
de Lacerda CF, Cambraia J, Oliva MA, Ruiz HA, Prisco JT (2003) Solute accumulation and distribution during shoot and leaf development in two sorghum genotypes under salt stress. Environ Exp Bot 49:107–120
de Lacerda CF, Cambraia J, Oliva MA, Ruiz HA (2005) Changes in growth and in solute concentrations in sorghum leaves and roots during salt stress recovery. Environ Exp Bot 54:69–76
Demiral T, Türkan I (2005) Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ Exp Bot 53:247–257
El Sabagh A, Islam MS, Skalicky M, AliRaza M, Singh K, AnwarHossain M, Arshad A (2021) Salinity stress in wheat (Triticum aestivum L.) in the changing climate: adaptation and management strategies. Front Agron 3:661932
Flowers TJ, Garcia A, Koyama M, Yeo AR (1997) Breeding for salt tolerance in crop plants the role of molecular biology. Acta Physiol Plant 19(4):427–433
Francois LE, Grieve CM, Maas EV, Lesch SM (1994) Time of salt stress affects growth and yield components of irrigated wheat. Agron J 86(1):100–107
Goudarzi M, Pakniyat H (2009) Salinity causes increase in proline and protein contents and peroxidase activity in wheat cultivars. J Appl Sci 9(2):348–353
Hamurcu M, Khan MK, Pandey A, Ozdemir C, Avsaroglu ZZ, Elbasan F, Gezgin S (2020a) Boron stress exposes differential antioxidant responses in maize cultivars (Zea mays L.). J Elementol 25:1291–1304
Hamurcu M, Khan MK, Pandey A, Ozdemir C, Avsaroglu ZZ, Elbasan F et al (2020b) Nitric oxide regulates watermelon (Citrullus lanatus) responses to drought stress. 3 Biotech 10:1–14
Hnilickova H, Kraus K, Vachova P, Hnilicka F (2021) Salinity stress affects photosynthesis, malondialdehyde formation, and proline content in Portulaca oleracea L. Plants 10(5):845
Islam MZ, Baset Mia MA, Islam MR, Akter A (2007) Effect of different saline levels on growth and yield attributes of mutant rice. J Soil Nat 1(2):18–22
Jakhar S, Mukherjee D (2014) Chloroplast pigments, proteins, lipid peroxidation and activities of antioxidative enzymes during maturation and senescence of leaves and reproductive organs of Cajanus cajan L. Physiol Mol Biol Plants 20:171–180
Jiang Y, Shiina T, Nakamura N, Nakahara A (2001) Electrical conductivity evaluation of postharvest strawberry damage. J Food Sci 66:1392–1395
Juan M, Rivero RM, Romero L, Ruiz JM (2005) Evaluation of some nutritional and biochemical indicators in selecting salt-resistant tomato cultivars. Environ Exp Bot 54(3):193–201
Kalhoro NA, Rajpar I, Kalhoro SA, Ali A, Raza S, Ahmed M, Kalhoro FA, Ramzan M, Wahid F (2016) Effect of salts stress on the growth and yield of wheat (Triticum aestivum L.). Am J Plant Sci 7:2257–2271
Kavii Kishor PB, Sreenivasulu N (2014) Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue? Plant Cell Environ 37:300–311
Khan MK, Pandey A, Hamurcu M, Avsaroglu ZZ, Ozbek M, Omay AH, Elbasan F, Omay MR, Gokmen F, Topal A, Gezgin S (2021) Variability in physiological traits reveals boron toxicity tolerance in aegilops species. Front Plant Sci 12:1–15
Kingsbury RW, Epstein E, Pearcy RW (1984) Physiological responses to salinity in selected lines of wheat. Plant Physiol 74:417–423
Kishor PBK, Sangam S, Amrutha RN, Laxmi PS, Naidu KR, Rao KRSS et al (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress toleranc. Curr Sci 88:424–438
Kumar SG, Reddy AM, Sudhakar C (2003) NaCl effects on proline metabolism in two high yielding genotypes of mulberry (Morus alba L.) with contrasting salt tolerance. Plant Sci 165:1245–1251
Liu D, Ford KL, Roessner U, Natera S, Cassin AM, Patterson JH, Bacic A (2013) Rice suspension cultured cells are evaluated as a model system to study salt responsive networks in plants using a combined proteomic and metabolomic profiling approach. Proteomics 13(12–13):2046–2062
Ma W, Mao Z, Yu Z, Mensvoort MV, Driessen P (2008) Effects of saline water irrigation on soil salinity and yield of winter wheat–maize in North China Plain. Irrig Drain Syst 22:3–18
Ma J, Du G, Li X, Zhang C, Guo J (2015) A major locus controlling malondialdehyde content under water stress is associated with fusarium crown rot resistance in wheat. Mol Genet Genom 290:1955–1962
Mahlooji M, Sharifi RS, Razmjoo J, Sabzalian MR, Sedghi M (2018) Effect of salt stress on photosynthesis and physiological parameters of three contrasting barley genotypes. Photosynthetica 56(2):549–556
Mahmoudi H, Kaddour R, Huang J, Nasri N, Olfa B, M’Rah S, Hannoufa A, Lachaâl M, Ouerghi Z (2011) Varied tolerance to NaCl salinity is related to biochemical changes in two contrasting lettuce genotypes. Acta Physiol Plant 33:1613–11622
Mandhania S, Madan S, Sawhney V (2006) Antioxidant defense mechanism under salt stress in wheat seedlings. Biol Plant 50:227–231
Mansour MMF, Salama KHA (2004) Cellular basis of salinity tolerance in plants. Environ Exp Bot 52:113–122
Menezes RV, Azevedo Neto ADD, Ribeiro MDO, Cova AMW (2017) "Growth and contents of organic and inorganic solutes in amaranth under salt stress. Pesqui Agropecuária Trop 47:22–30
Oraby HF, Ransom CB, Kravchenko AN, Sticklen MB (2005) Barley HVA1 gene confers salt tolerance in R3 transgenic oat. Crop Sci 45:2218–2227
Parida AK, Das AB, Mittra B (2004) Effects of salt on growth, ion accumulation photosynthesis and leaf anatomy of the man- grove, Bruguiera parviflora. Trees 18:167–174
Plaut Z, Edelstein M, Ben-Hur M (2013) Overcoming salinity barriers to crop production using traditional methods. Crit Rev Plant Sci 32:250–291
Puvanitha S, Mahendran S (2017) Effect of salinity on plant height, shoot and root dry weight of selected rice cultivars. Sch J Agric Vet Sci 4(4):126–131
Rahneshan Z, Nasibi F, Moghadam AA (2018) Effects of salinity stress on some growth, physiological, biochemical parameters and nutrients in two pistachio (Pistacia vera L.) rootstocks. J Plant Interact 13:73–82
Sahin U, Ekinci M, Ors S, Turan M, Yildiz S, Yildirim E (2018) Effects of individual and combined effects of salinity and drought on physiological, nutritional and biochemical properties of cabbage (Brassica oleracea var. capitata). Sci Hortic 240:196–204
Shaheen S, Naseer S, Ashraf M, Akram NA (2013) "Salt stress affects water relations, photosynthesis, and oxidative defense mechanisms in Solanum melongena L. J Plant Interact 8:85–96
Shalhevet J, Huck MG, Schroeder BP (1995) Root and shoot growth responses to salinity in maize and soybean. Agron J 87(3):512–516
Szabados L, Savouré A (2010) Proline: a multifunctional amino acid. Trends Plant Sci 15:89–97
Turan MA, Elkarim AHA, Taban N, Taban S (2009) Effect of salt stress on growth, stomatal resistance, proline and chlorophyll concentrations on maize plant. Afr J Agric Res 4:893–897
Vatanfada J, Najafi A (2011) Environmental challenges in trans-boundary waters, case study: hamoon hirmand wetland (Iran and Afghanistan). Intl J Water Resour Arid Environ 1(1):16–24
Yazici I, Türkan I, Sekmen AH, Demiral T (2017) Salinity tolerance of purslane (Portulaca oleracea L.) is achieved by enhanced antioxidative system, lower level of lipid peroxidation and proline accumulation. Environ Exp Bot 61:49–57
Zeeshan M, Lu M, Sehar S, Holford P, Wu F (2020) Comparison of biochemical, anatomical, morphological, and physiological responses to salinity stress in wheat and barley genotypes deferring in salinity tolerance. Agronomy 127(10):1–15
Acknowledgements
The authors thank the ministry of agriculture, irrigation and livestock (MAIL) of Afghanistan for providing seeds of the two winter wheat cultivars.
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MWA wrote the paper and measured all physiological and biochemical measurements. SA did data analysis and interpretation/discussion of data, AFS collected agronomical data and participated in the discussion and writing of the paper. All authors read and approved the final manuscript.
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Amin, M.W., Aryan, S. & Samadi, A.F. Interpretation of morpho-physiological and biochemical responses of winter wheat under different sodium chloride concentrations. J. Crop Sci. Biotechnol. 26, 563–571 (2023). https://doi.org/10.1007/s12892-023-00200-9
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DOI: https://doi.org/10.1007/s12892-023-00200-9