Abstract
Brachypodium distachyon has been highlighted as a model monocot plant with small genome and short life cycle. Biofuels are being developed as renewable energy sources to replace fossil fuels. Bioethanol production is negatively correlated with lignin content. Here, Brachypodium was acutely or chronically irradiated at doses of 50, 100, 150, 200, and 250 Gy. The effect of radiation on plant growth and generation of mutant populations was explored. The lethal effect of radiation was higher in acutely irradiated M0 populations. A dose-dependent negative effect in plant height, tiller number, floral spikelet, and total seed number was observed, with a positive effect in days to heading. The phenotype of 1,773 M1 plants was evaluated, with 417 plants being selected to construct the M2 population. The 31 M2 plants that showed the least staining with phloroglucinol were selected. These mutants could be useful materials for studies such as identification of nucleotide substitutions in genes involved in lignin biosynthesis pathway, monitoring of mutant physiological traits, and evaluation of fitness for bioethanol production. As biological resources, the M2 populations generated in this work will contribute to studies of functional genomics of Brachypodium and to the breeding of grass crops.
Similar content being viewed by others
References
Casler MD, Pedersen JF, Undersander DJ. 2003. Forage yield and economic losses associated with the brownmidrib trait in sudangrass. Crop Sci. 43: 782–789
Chapple C, Ladisch M, Melian R. 2007. Loosening lignin’s grip on biofuel production. Nat. Biotechnol. 25: 746–747
Chen F, Dixon RA. 2007. Lignin modification improves fermentable sugar yields for biofuel production. Nat. Biotechnol. 25: 759–761
Do CT, Pollet B, Thévenin J, Sibout R, Denoue D, Barrière Y, Lapierre C, Jouanin L. 2007. Both caffeoyl Coenzyme A 3-O-methyltransferase 1 and caffeic acid O-methyltransferase 1 are involved in redundant functions for lignin, flavonoids and sinapoyl malate biosynthesis in Arabidopsis. Planta 226: 1117–1129
Donini P, Sonnino A. 1998. Induced mutation in plant breeding: current status and future outlook. In SM Jain, DS Brar, BS Ahloowalia, eds, Somaclonal variation and induced mutation in crop improvement, Kluwer Academic Publishers, London, pp 255–291
Elkind Y, Edwards R, Mavandad M, Hedrick SA, Ribak O, Dixon RA, Lamb CJ. 1990. Abnormal plant development and down-regulation of phenylpropanoid biosynthesis in transgenic tobacco containing a heterologous phenylalanine ammonia-lyase gene. Proc. Natl. Acad. Sci. USA. 87: 9057–9061
Fu HW, Li YF, Shu QY. 2008. A revisit of mutation induction by gamma rays in rice (Oryza sativa L.) implications of microsatellite markers for quality control. Mol. Breed. 22: 281–288
Garvin DF. 2007. Brachypodium: a new monocot model plant system emerges. J. Sci. Food Agric. 87: 1177–1179
Gray KA, Zhao L, Emptage M. 2006. Bioethanol. Curr. Opin. Chem. Biol. 10: 141–146
Guillon F, Bouchet B, Jamme F, Robert P, Quéméner B, Barron C, Larré C, Dumas P, Saulnier L. 2011. Brachypodium distachyon grain: characterization of endosperm cell walls. J. Exp. Bot. 62: 1001–1015
Hisano H, Nandakumar R, Wang ZY. 2009. Genetic modification of lignin biosynthesis for improved biofuel production. In Vitro Cell. Dev. Biol. 45: 306–313
José MFM, Manuel M, Jorge O, Rafael G. 1997. Sunflower mutant containing high levels of palmitic acid in high oleic background. Euphytica 97: 113–117
Keating JD, Panganiban C, Mansfield SD. 2006. Tolerance and adaptation of ethanologenic yeasts to lignocellulosic inhibitory compounds. Biotechnol. Bioeng. 93: 1196–1206
Kellogg EA. 2001. Evolutionary history of the grasses. Plant Physiol. 125: 1198–1205
Leplé JC, Dauwe R, Morreel K, Storme V, Lapierre C et al. 2007. Downregulation of cinnamoyl-coenzyme A reductase in poplar: multiple-level phenoty** reveals effects on cell wall polymer metabolism and structure. Plant Cell 19: 3669–3691
Li X, Weng JK, Chapple C. 2008. Improvement of biomass through lignin modification. Plant J. 54: 569–851
Lorenz AJ, Anex RP, Isci A, Coors JG, de Leon N, Weimer PJ. 2009. Forage quality and composition measurements as predictors of ethanol yield from maize (Zea mays L.) stover. Biotechnol. Biofuels. 2: 5
Luckey TD. 1982. Hormesis with ionizing radiation. Nucl. Sci. Eng. 82: 365–366
Martínez JMF, Mancha M, Osorio J, Garcés R. 1997. Sunflower mutant containing high levels of palmitic acid in high oleic background. Euphytica 97: 113–116
Matsukura C, Yamaguchi I, Inamura M, Ban Y, Kobayashi Y, Yin YG, Saito T, Kuwata C, Imanishi S, Nishimura S. 2007. Generation of gamma irradiation-induced mutant lines of the miniature tomato (Solanum lycopersicum L.) cultivar ‘Micro-Tom’. Plant Biotechnol. 24: 39–44
Mir Derikvand M, Sierra JB, Ruel K, Pollet B, Do CT, Thévenin J, BuVard D, Jouanin L, Lapierre C. 2008. Redirection of the phenylpropanoid pathway to feruloyl malate in Arabidopsis mutants deficient for cinnamoyl-CoA reductase 1. Planta 227: 943–956
Monties B. 1989. Lignins. In PP Dey, JB Harborne, eds, Methods in Plant Biochemistry, Academic Press, London, pp 113–157
Opanowicz M, Hands P, Betts D, Parker ML, Toole GA, Mills ENC, Doonan JH, Drea S. 2011. Endosperm development in Brachypodium distachyon. J. Exp. Bot. 62: 735–748
Pedersen JF, Vogel KP, Funnell DL. 2005. Impact of reduced lignin on plant fitness. Crop Sci. 45: 812–819
Pincon G, Maury S, Hoffmann L, Geoffroy P, Lapierre C, Pollet B, Legrand M. 2001. Repression of O-methyltransferase genes in transgenic tobacco affects lignin synthesis and plant growth. Phytochemistry 57: 1167–1176
Pomar F, Merino F, Ros Barceló A. 2002. O-4-Linked coniferyl and sinapyal aldehydes in lignifying cell walls are the main targets of the Wiesner (phloroglucinol-HCL) reaction. Protoplasma 220: 17–28
Ragauskas AJ, Williams CK, Davison BH, Britovsek G, Cairney J, et al. 2006. The path forward for biofuels and biomaterials. Science 311: 484–489
Ralph J, Akiyama T, Kim H, Lu F, Schatz PF, Marita JM, Ralph SS, Reddy MSS, Chen F, Dixon RA. 2006. Effects of coumarate 3-hydroxylase down-regulation on lignin structure. J. Biol. Chem. 281: 8843–8853
Reddy MSS, Chen F, Shadle G, Jackson L, Aljoe H, Dixon RA. 2005. Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.). Proc. Natl. Acad. Sci. USA. 102: 16573–16578
Schilmiller AL, Stout J, Weng JK, Humphreys J, Ruegger MO, Chapple C. 2009. Mutations in the cinnamate 4-hydroxylase gene impact metabolism, growth and development in Arabidopsis. Plant J. 60: 771–782
Shadle G, Chen F, Reddy MSS, Jackson L, Nakashima J, Dixon RA. 2007. Down-regulation of hydroxycinnamoyl CoA: Shikimate hydroxycinnamoyl transferase in transgenic alfalfa affects lignification, development and forage quality. Phytochemistry 68: 1521–1529
Shah SM, Sharif M. 1994. Studies on the effect of gamma radiation on some morphological characters of mungbean (Vigna radiata (L.) Wilczek) cultivars. Sci. Khyber. 7: 37–44
Shapiro SS, Wilk MB. 1965. An analysis of variance of normality (complete samples). Biometrika 52: 591–611
Sparrow AH, Woodwell GM. 1962. Prediction of the sensitivity of plants to chronic gamma irradiation. Radiat. Bot. 2: 9–26
Speer EO. 1987. A method of retaining phloroglucinol proof lignin. Stain Technol. 62: 279–280
Stadler LJ. 1928. Mutations in barley induced by X-rays and radium. Science 68: 186–187
Tanaka A, Shikazono N and Hase Y. 2010. Studies on biological effects of ion beams on lethality, molecular nature of mutation, mutation rate, and spectrum of mutation phenotype for mutation breeding in higher plants. J. Radiat. Res. 51: 223–233
Torney F, Moeller L, Scarpa A, Wang K. 2007. Genetic engineering approaches to improve bioethanol production from maize. Curr. Opin. Biotech. 18: 193–199
Wang Y, Wang F, Zhai H, Liu Q. 2007. Production of a useful mutant by chronic irradiation in sweet potato. Sci. Hortic. 111: 173–178
Watt M, Schneebeli K, Dong P, Wilson LW. 2009. The shoot and root growth of Brachypodium and its potential as a model for wheat and other cereal crops. Funct. Plant Biol. 36: 960–969
Yuan JS, Tiller KH, Al-Ahmad H, Stewart NR, Stewart NC. 2008. Plants to power: bioenergy to fuel the future. Trends Plant Sci. 13: 421–429
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, M.B., Kim, D.Y., Jeon, W.B. et al. Effect of gamma radiation on growth and lignin content in Brachypodium distachyon . J. Crop Sci. Biotechnol. 16, 105–110 (2013). https://doi.org/10.1007/s12892-013-0022-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12892-013-0022-9