Abstract
The Asian planthopper Ricania speculum is a major agricultural pest for a variety of crops in tropical and subtropical areas. Rapid species diagnosis and source determination of this pest would be essential to the formulation of effective management, control and prevention strategy, and mitochondrial DNA has proven powerful for such purposes. In this study, we assembled and characterized its complete mitochondrial genome from Illumina sequencing reads. The whole genome of R. speculum is 15,729 bp in length, and encodes the typical set of 37 mitochondrial genes (incl. 13 PCGs, 22tRNAs and 2 rRNAs) and a control region. All PCGs are initiated with the typical ATN codons, and are terminated with either the complete TAA/TAG codons or the incomplete T(aa) codon. The nucleotide composition is asymmetric (47.4 %A, 14.8 %C, 9.5 %G and 28.3 %T) with an obvious bias towards A and T. Phylogenetic analysis indicated that R. speculum is closely related to Ricania marginalis.
This is a preview of subscription content, log in via an institution to check access.
References
Bernt M, Donath A, Jühling F, Externbrink F, Florentz C, Fritzsch G, Pütz J, Middendorf M, Stadler PF (2013) MITOS: improved de novo metazoan mitochondrial genome annotation. Mol Phylogenet Evol 69:313–319. doi:10.1016/j.ympev.2012.08.023
Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114–2120. doi:10.1093/bioinformatics/btu170
Hahn C, Bachmann L, Chevreux B (2013) Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads—a baiting and iterative map** approach. Nucl Acids Res 41:e129. doi:10.1093/nar/gkt371
Li J-L, Shao Y-H, Tang J-C, Li J-X, Tang H, Li X-D (2011) The spatial distribution and the sampling technique of adult Ricania speculum (Walker). Guangdong Agric Sci 2011:81–83. doi:10.16768/j.issn.1004-874x.2011.19.042
Mazza G, Pennacchio F, Gargani E, Franceschini I, Roversi PF, Cianferoni F (2014) First report of Ricania speculum (Walker, 1851) in Europe (Hemiptera: Fulgoromorpha: Ricaniidae). Zootaxa 3861:297–300. doi:10.11646/zootaxa.3861.3.7
Ohtsuki T, Kawai G, Watanabe K (2002) The minimal tRNA: unique structure of Ascaris suum mitochondrial tRNASerUCU having a short T arm and lacking the entire D arm. FEBS Lett 514:37–43. doi:10.1016/S0014-5793(02)02328-1
Rossi E, Lucchi A (2015) The Asian planthopper Ricania speculum (Walker) (Homoptera: Ricaniidae) on several crops in Italy: a potential threat to the EPPO region? EPPO Bull 45:119–122. doi:10.1111/epp.12175
Song N, Liang A-P, Ma C (2010) The complete mitochondrial genome sequence of the planthopper, Sivaloka damnosus. J Insect Sci 10:76. doi:10.1673/031.010.7601
Song N, Liang A-P, Bu C-P (2012) A molecular phylogeny of Hemiptera inferred from mitochondrial genome sequences. PLoS One 7:e48778. doi:10.1371/journal.pone.0048778
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. doi:10.1093/molbev/mst197
Xu H, Liu Z, Bao Q, Zeng L, Jiang S (2013) Damage and control of Ricania speculum in mangrove in Futian, Shenzhen. Guangdong Sci Tech 29:26–30
Acknowledgments
This work was financially supported by the Natural Science Basic Research Plan in Shaanxi Province (2013JC2-04; 2013JC2-05) and the Key Research Fund of Shaanxi Academy of Sciences (2012K-01). The authors thank Zeng-Qiang Qian for his assistance in manuscript preparation.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Zhang, QX., Guan, DL., Niu, Y. et al. Characterization of the complete mitochondrial genome of the Asian planthopper Ricania speculum (Hemiptera: Fulgoroidea: Ricannidae). Conservation Genet Resour 8, 463–466 (2016). https://doi.org/10.1007/s12686-016-0600-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-016-0600-5