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Green Synthesis, Characterization and In Vivo Evaluation of White Tea Silver Nanoparticles with 5-Fluorouracil on Colorectal Cancer

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Abstract

White tea (WT) has the highest amount of catechin which previously was found to enhance anti-cancer activity. To enhance the bioavailability of WT, silver nanoparticle (AgNP) was used to synthesize WT nanoparticle (WTNP). This present study aimed to determine the effectiveness of WTNP alone and co-administered with 5FU in the prophylactic study. WTNP was characterized by UV–Vis spectra, X-ray diffraction (XRD) spectra, transmission electron microscope (TEM) and Fourier-transform infrared spectroscopy (FTIR) and tested for their acute toxicity. In animal studies, all groups except the naïve group were given once a week 65 mg/kg of 1,2-dimethylhydrazine (DMH) subcutaneously, intraperitoneal injection of 50 mg/kg 5FU and daily administration of DMH 50 mg/kg WT and 5FU WTNPs. Aberrant crypt foci (ACF) and histology were determined and analysed. WTNP showed circular and pseudospherical shaped and 25–50 nm size range with no acute toxicity observed on rats. The DMH group was the highest in average ACF count (37.3 ± 3.18), which ACF was shown to decrease using the treatment, WT group (16.67 ± 3.28), 5FU monotherapy (5.67 ± 2.19) and WTFU (6.33 ± 1.33). The highest AC frequency was found at the DMH group (24.67 ± 1.20, 12.67 ± 1.45) compared to other groups. The chemo-suppressive effect of WTNP and WTFU combined effects was observed in the present study. WTNP expresses anti-cancer properties; however, it does not produce any synergistic effect.

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Authors and Affiliations

  1. PAPRSB Institute of Health Sciences, Universiti Brunei Darussalam, Jalan Tungku Link BE1410, Bandar Seri Begawan, Brunei Darussalam

    Sheba R. David, Khairunnasibah Abdullah & Rajan Rajabalaya

  2. Department of Pharmacology, SIMATS, Saveetha Dental College and Hospitals, Saveetha University, Chennai, 600077, TN, India

    Rajeshkumar Shanmugam & Lakshmi Thangavelu

  3. Institute of Pharmacy, Jalpaiguri, West Bengal, India

    Sanjoy Kumar Das

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  1. Sheba R. David
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Contributions

Dr Sheba designed the experiments and organized the execution of the studies. Dr Rajan designed the experiments for the preparation of the extracts and the nanoparticle preparation. Ms. Khairunnasibah performed the experiments. The rest of the authors contributed equally to the studies.

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Correspondence to Rajan Rajabalaya.

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The research ethics for using animals were reviewed and approved by the University Research Ethics Committee (UREC), Universiti Brunei Darussalam (Ref: UBD/OAVCR/UREC/Dec18-02). Either gender of 2–3-month-old Wistar rats, which initially weighed 230–280 g, was procured from the animal house of the Institute of Health Science, Universiti Brunei Darussalam. Food and water were provided ad libitum and housed under standard conditions (27 ± 2 °C; 70–80% humidity; and 12 h light/12 h darkness cycle) with a minimum of 5 days for acclimatization to housing condition, prior to the intervention.

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David, S.R., Abdullah, K., Shanmugam, R. et al. Green Synthesis, Characterization and In Vivo Evaluation of White Tea Silver Nanoparticles with 5-Fluorouracil on Colorectal Cancer. BioNanoSci. 11, 1095–1107 (2021). https://doi.org/10.1007/s12668-021-00905-7

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