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Evaluation of Expression of WNT1 and PTCH Genes in Peripheral Blood of Patients with Odontogenic Cysts and Tumours of the Jaws by Quantitative RT-PCR: A Pilot Study

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Abstract

Introduction

Odontogenic lesions of the maxillofacial region constitute a complex group of lesions with diverse histopathologic types and clinical behaviour. Early diagnosis is important to minimize the need for radical surgery and to improve quality of life of the patients. Tumour markers play an essential role in the molecular level understanding of Odontogenic lesions and also used for early diagnosis and target therapies which improves the quality of life of the patients. Patched, a tumour suppressor gene encodes the transmembrane protein PTCH and is a receptor for the morphogen Sonic Hedgehog. It is evident that PTCH gene mutations occur in odontogenic keratocysts and the Hedgehog signalling pathway has an important role during tooth formation. WNT 1 is a key signal molecule that controls cell growth and proliferation. WNT pathway abnormalities are reported to induce tumour occurrence. Hence, my study was to determine the presence of WNT1 and PTCH in peripheral blood of patients with Odontogenic lesions using quantitative RT-PCR.

Materials and Methods

In this cross-sectional study, two groups were included: Group 1—blood samples from 8 individuals with odontogenic cysts and tumours, and Group 2—blood samples of 8 individuals without Odontogenic lesions. 2 ml of blood sample was collected from radial veins into PAX gene tubes containing RNA stabilizing agent and stored at a temperature of 2 to 4 degrees and transported to Enable Biolabs India Pvt Ltd., Chennai. PAX gene tubes were subjected to centrifugation at 8000 rpm to separate plasma fraction. Reverse transcription of mRNA was performed using miScript II RT Kit (Cat#218161, Qiagen, Germany) to synthesize cDNA. GAPDH house-kee** gene used as control.

Results

The study group had 3 males and 5 females (n = 8) with a mean age group of 32.6 years and the control group had 2 males and 6 females (n = 8) with mean age of 35.2 years. Group I (study group) showed 37.5% positive expression of WNT1 gene with a p value of 0.055 (p > 0.05) and 50% positive expression of PTCH with a p value of 0.021 (p < 0.05) (Figs. 3 and 4) which was statistically significant when compared with control group. Group II (control group) showed 100% negative expression for WNT1 and PTCH genes.

Conclusion

WNT1 and PTCH genes were expressed in peripheral blood of patients with odontogenic lesions. WNT1 and PTCH genes may be potential predictors in individuals who would develop odontogenic lesions. Further studies on expression of WNT1 and PTCH genes with larger number of samples might give a future scope for target therapy in odontogenic lesions.

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References

  1. Lench NJ, High AS, Markham AF et al (1996) Investigation of chromosome 9q22.3-q31 DNA marker loss in odontogenic keratocysts. Eur J Cancer B Oral Oncol 32:202–206

    Article  Google Scholar 

  2. Blotta S, Jakubikova J, Calimeri T, Roccaro AM, Amodio N, Azab AK, Foresta U, Mitsiades CS, Rossi M, Todoerti K et al (2012) Canonical and noncanonical hedgehog pathway in the pathogenesis of multiple myeloma. Blood 120:5002–5013

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Thesleff I, Sharpe P (1997) Signalling networks regulating dental development. Mech Dev 67:111–123

    Article  CAS  PubMed  Google Scholar 

  4. ** Lu, Yang J et al (2009) Advances of Wnt signalling pathway in dental development and potential clinical application. Organogenesis 00:1–10

    Google Scholar 

  5. Croce JC, McClay DR (2009) Evolution of the Wnt pathways. Wnt Signal 3–18

  6. Tamura M, Nemoto E, Sato MM, Nakashima A, Shimauchi H (2010) Role of the Wnt signaling pathway in bone and tooth. Front Biosci (Elite Ed) 2:1405–1413

    Article  PubMed  Google Scholar 

  7. Nusse R, Varmus HE (1982) Many tumors induced by the mouse mam-mary tumor virus contain a provirus integrated in the same region of the host genome. Cell 31:99–109

    Article  CAS  PubMed  Google Scholar 

  8. Rijsewijk F, Schuermann M, Wagenaar E, Parren P, Weigel D, Nusse R (1987) The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wing-less. Cell 50:649–657

    Article  CAS  PubMed  Google Scholar 

  9. Chuah KS, Siar CH et al (2009) Wingless type protein-1(Wnt-1) expression in primary conventional unicystic ameloblastoma and their recurrent tumours. J Hard Tissue Biol 18:63–70

    Article  Google Scholar 

  10. Siar CH, Nagatsuka H, Han PP, Buery RR, Tsujigiwa H, Nakano K, Ng KH, Kawakami T (2012) Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma. J Oral Pathol Med 41(4):332–339

    Article  PubMed  Google Scholar 

  11. Dutra S-N, Pire F-R et al (2017) Immunoexpression of Wnt/β-catenin signaling pathway proteins in ameloblastoma and calcifying cystic odontogenic tumor. J Clin Exp Dent 9:e136–e140

    PubMed  PubMed Central  Google Scholar 

  12. Wang G-N, Zhong M et al (2018) Expression of WNT1 in ameloblastoma and its significance. Oncol Lett 16:1507–1512

    PubMed  PubMed Central  Google Scholar 

  13. Hahn H, Wicking C, Zaphiropoulos PG, Gailani MR, Shanley S, Chidambaram A, Vorechovsky I, Holmberg E, Unden AB, Gillies S, Negus K, Smyth I, Pressman C, Leffell DJ, Gerrard B, Goldstein AM, Dean M, Toftgard R, Chenevix-Trench G, Wainwright B, Bale AE (1996) Mutations of the human homolog of Drosophila patched in the nevoid basal cell carcinoma syndrome. Cell 85:841–851

    Article  CAS  PubMed  Google Scholar 

  14. Johnson RL, Rothman AL, **e J, Goodrich LV, Bare JW, Bonifas JM, Quinn AG, Myers RM, Cox DR, Epstein EH Jr, Scott MP (1996) Human homolog of patched, a candidate gene for the basal cell nevus syndrome. Science 272:1668–1671

    Article  CAS  PubMed  Google Scholar 

  15. Stone DM, Hynes M, Armanini M, Swanson TA, Gu Q, Johnson RL, Scott MP, Pennica D, Goddard A, Phillips H, Noll M, Hooper JE, de Sauvage F, Roserthal A (1996) The tumour suppressor gene patched encodes a candidate receptor for Sonic hedgehog. Nature 384:129–134

    Article  CAS  PubMed  Google Scholar 

  16. Vered M, Peleg O et al (2009) The immunoprofile of odontogenic keratocyst (keratocystic odontogenic tumor) that includes expression of PTCH, SMO, GLI-1 and bcl-2 is similar to ameloblastoma but different from odontogenic cysts. J Oral Pathol Med 38:597–604

    Article  CAS  PubMed  Google Scholar 

  17. Barreto DC, Gomez RS et al (2000) PTCH gene mutations in odontogenic keratocysts. J Dent Res 79:1418

    Article  CAS  PubMed  Google Scholar 

  18. Pavelic B, Levanat S, Crnic I, Kobler P, Anic I, Manojlovic S et al (2001) PTCH gene altered in dentigerous cysts. J Oral Pathol Med 30:569–576

    Article  CAS  PubMed  Google Scholar 

  19. Li TJ, Yuan J-W et al (2008) PTCH germline mutations in Chinese naevoid basal cell carcinoma syndrome patients. Oral Dis 14:174–179

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Vivekananda Dental College for Women, Thiruchengodu, Tamilnadu, India

    Srinath Kalyanasundaram & Saravanan Kandasamy

  2. Vinayaka Mission’s Sankarachariyar Dental College, Vinayaka Missions Research Foundation (Deemed to be University), Salem, Tamilnadu, India

    Reena Rachel John

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  1. Srinath Kalyanasundaram
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  2. Saravanan Kandasamy
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Correspondence to Srinath Kalyanasundaram.

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Kalyanasundaram, S., Kandasamy, S. & John, R.R. Evaluation of Expression of WNT1 and PTCH Genes in Peripheral Blood of Patients with Odontogenic Cysts and Tumours of the Jaws by Quantitative RT-PCR: A Pilot Study. J. Maxillofac. Oral Surg. 22, 1123–1129 (2023). https://doi.org/10.1007/s12663-023-02014-2

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