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Enhanced Biogas Production from Thermophilic Anaerobic Digestion of Poultry Slaughterhouse Sludge: Effect of Thermal Pretreatment and Micronutrients Supplementation

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Abstract

Poultry slaughter effluents contain biodegradable organics, which include blood, feathers, viscera, soft tissues removed during trimming and cutting, bones, and soil. Slaughterhouse sludge usually contains many of these compounds and a high concentration of pathogenic microorganisms. In this study, the influence of thermal pretreatment on the conversion of mesophilic anaerobic digestion (MAD) to thermophilic anaerobic digestion (TAD) was evaluated, and micronutrient supplementation (Fe, Co, Ni, and Mo) to improve the production of biogas from poultry slaughter sludge. Six experimental stages were carried out, sludge sampling and characterization, application of thermal pretreatment, the adaptation of the mesophilic to thermophilic process, operation of TAD with increased organic loading rate (OLR), selection of micronutrient doses, and semicontinuous operation of TAD with micronutrients. The application of thermal pretreatment led to notable increases in the levels of carbohydrates, lipids, and soluble proteins, and a high inactivation of pathogens was obtained. The adaptation process from MAD to TAD required equidistant increments in temperature (+ Δ5 °C) and operation time (Δ15 days), resulting in a cumulative biogas production of 144.5 L. The TAD system effectively accommodated an OLR of 5 kgVS/(m3 d) without encountering inhibition. Moreover, the initial dose of micronutrients administered during the semicontinuous operation resulted in a 16% increase in biogas production compared to the control. Analysis of kinetic parameters using the modified Gompertz equation consistently yielded R2 values above 0.96 in all experimental stages. Overall, the findings highlight the positive impact of micronutrient supplementation and thermal pretreatment on biogas production within an enhanced TAD system.

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Data Availability

The datasets generated during and analysed during the current study is confidential but are available from the corresponding author on reasonable request.

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Acknowledgements

The authors acknowledge support from Tecnológico Nacional de México Campus Orizaba.

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Authors and Affiliations

  1. Tecnológico Nacional de México, Instituto Tecnológico de Orizaba, Oriente 9, Colonia Emiliano Zapata, 94320, Orizaba, Veracruz, México

    Juan Manuel Méndez-Contreras

  2. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Unidad Noreste, Autopista Monterrey-Aeropuerto, Vía de La Innovación 404, Parque PIIT, 66628, Apodaca, Nuevo León, México

    Noemi Nava-Valente

  3. Tecnológico Nacional de México, Instituto Tecnológico Superior de Huatusco, Avenida 25 Poniente 100, Colonia Reserva Territorial, CP 94100, Huatusco, Veracruz, México

    Jesús Atenodoro-Alonso

  4. Universidad Politécnica de Huatusco, Calle 22 Sur S/N Colonia Reserva Territorial, CP 94106, Huatusco, Veracruz, México

    Luis Antonio López-Escobar

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  1. Juan Manuel Méndez-Contreras
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  2. Jesús Atenodoro-Alonso
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Contributions

All authors contributed to the study conception and design. JMM-C, JA-A, LALE, and NN-V: Material preparation, data collection and analysis were performed. JMM-C and NN-V: The first draft of the manuscript was written and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Noemi Nava-Valente.

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Méndez-Contreras, J.M., Atenodoro-Alonso, J., López-Escobar, L.A. et al. Enhanced Biogas Production from Thermophilic Anaerobic Digestion of Poultry Slaughterhouse Sludge: Effect of Thermal Pretreatment and Micronutrients Supplementation. Waste Biomass Valor 15, 2201–2214 (2024). https://doi.org/10.1007/s12649-023-02277-3

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