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Valorization of Sugarcane Bagasse into Fermentable Sugars by Efficient Fungal Cellulolytic Enzyme Complex

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Abstract

The study aimed to identify an efficient cellulase producing fungus and to evaluate its potential for saccharification of sugarcane bagasse. A total of 17 fungi were isolated and among these, five isolates (JS4, JS5, JS6, JS8 and JS17) showed high cellulolytic index and were tentatively identified to be belonging to genera, i.e., Aspergillus and Penicillium. The fungal isolate JS7 exhibited significantly high total cellulase (43.52 U/L), endoglucanase (171.98 U/L) and β-glucosidase (78.65 U/L) activities, when grown under liquid shake flask conditions. BLAST analysis, multiple sequences and phylogenetic analysis showed that the isolate JS7 was highly similar with fungal strain Penicillium mallochii from gene bank. The enzyme extract from P. mallochii was concentrated using tangential ultrafiltration with 67.39% recovery of filter paper activity (total cellulase activity) and 1.75-fold purification. The saccharification of alkali pre-treated sugarcane bagasse with cellulase resulted in maximum reducing sugar yield of 651.60 mg/g with saccharification efficiency of 78.29%.

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The datasets generated during and/or analyzed during this study are included in this published article.

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Acknowledgements

Authors are thankful to Head, Department of Renewable Energy Engineering, P.A.U., Ludhiana for providing instrumentation facilities to pursue this research work.

Funding

The authors are thankful for the grants received from the DST-INSPIRE (Innovation in Science Pursuit for Inspired Research), Department of Science and Technology, Government of India.

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Authors and Affiliations

  1. Department of Biochemistry, Punjab Agricultural University, Ludhiana, Punjab, 141 004, India

    Jaspreet Kaur

  2. Department of Renewable Energy Engineering, Punjab Agricultural University, Ludhiana, Punjab, 141 004, India

    Monica Sachdeva Taggar

  3. Department of Soil Science, Punjab Agricultural University, Ludhiana, Punjab, 141 004, India

    Anu Kalia

  4. Regional Research Station, Punjab Agricultural University, Kapurthala, Punjab, 144 601, India

    Gulzar Singh Sanghera

  5. Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab, 141 004, India

    Gurvinder Singh Kocher

  6. Department of Mathematics, Statistics & Physics, Punjab Agricultural University, Ludhiana, Punjab, 141 004, India

    Mohammed Javed

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  1. Jaspreet Kaur
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Contributions

JK has performed the experiments, analysed and written the manuscript. MST has conceptualized, supervised the study and edited the manuscript. AK and GSK have supervised the microbiological studies. GSS has provided the biomass of sugarcane for conducting the experiments. MJ has been involved in the statistical analysis of the data. All authors have read and approved the final manuscript.

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Correspondence to Monica Sachdeva Taggar.

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Kaur, J., Taggar, M.S., Kalia, A. et al. Valorization of Sugarcane Bagasse into Fermentable Sugars by Efficient Fungal Cellulolytic Enzyme Complex. Waste Biomass Valor 14, 963–975 (2023). https://doi.org/10.1007/s12649-022-01918-3

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