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Protecting Effect of Bacillus coagulans T242 on HT-29 Cells Against AAPH-Induced Oxidative Damage

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Abstract

The aim of the present study was to investigate the in vitro antioxidant potential of Bacillus coagulans T242. B. coagulans T242 showed better antioxidant activities, including the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging ability, lipid peroxidation inhibiting ability and reducing ability, than those exerted by Lactobacillus rhamnosus GG (LGG). B. coagulans T242 positively regulated the expression of the nuclear factor erythroid 2-relatedfactor 2/Kelch-like ECH-associated protein-1 (Nrf2/Keap1) pathway-related proteins (Nrf2, Keap1, heine oxygenase-1 (HO-1)); increased antioxidant enzymes (glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD)); reduced the content of malondialdehyde (MDA) level; decreased the expression of inflammatory-related cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α); and thus increased the survival rate in 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH)-damaged HT-29 cells. This study proved that B. coagulans T242 exerted antioxidative effects by quenching oxygen free radicals and activating the Nrf2 signaling pathway in HT-29 cells.

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Data Availability

The datasets generated or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This research was supported by Liaoning Province Natural Science Foundation of China (2020-MZLH-39), scientific research fund project of Liaoning Provincial Department of Education (J2019017).

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  1. School of Food Science and Technology, Dalian Polytechnic University, No. 1 Qinggongyuan, Gan**gzi District, Liaoning, 116034, People’s Republic of China

    **aoxi Gao, Yuhong Zhang, Guangqing Mu, Yunpeng Xu, **nmiao Wang, Yanfeng Tuo & Fang Qian

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Contributions

**aoxi Gao and Yuhong Zhang performed the experiments and wrote the manuscript. Yunpeng Xu, **nmiao Wang checked the logicality and language of this manuscript. Fang Qian, Yanfeng Tuo and Guangqing Mu conceived and designed the study. Fang Qian was responsible for overall study coordination of this manuscript.

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Correspondence to Yanfeng Tuo or Fang Qian.

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Gao, X., Zhang, Y., Mu, G. et al. Protecting Effect of Bacillus coagulans T242 on HT-29 Cells Against AAPH-Induced Oxidative Damage. Probiotics & Antimicro. Prot. 14, 741–750 (2022). https://doi.org/10.1007/s12602-022-09917-5

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