Abstract
Background
To describe mumps virus (MuV) used as a vector to express enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) genes.
Methods
Molecular cloning technique was applied to establish the cDNA clones of recombinant mumps viruses (rMuVs). rMuVs were recovered based on our reverse genetic system of MuV-S79. The properties of rMuVs were determined by growth curve, plaque assay, fluorescent microscopy and determination of fluorescent intensity.
Results
Three recombinant viruses replicated well in Vero cells and similarly as parental rMuV-S79, expressed heterologous genes in high levels, and were genetically stable in at least 15 passages.
Conclusion
rMuV-S79 is a promising platform to accommodate foreign genes like marker genes, other antigens and immunomodulators for addressing various diseases.
References
Escriou N, Callendret B, Lorin V, Combredet C, Marianneau P, Fevrier M, et al. Protection from SARS coronavirus conferred by live measles vaccine expressing the spike glycoprotein. Virology. 2014;452–453:32–41.
Veinalde R, Grossardt C, Hartmann L, Bourgeois-Daigneault MC, Bell JC, Jager D, et al. Oncolytic measles virus encoding interleukin-12 mediates potent antitumor effects through T cell activation. Oncoimmunology. 2017;6:e1285992.
Gupta G, Giannino V, Rishi N, Glueck R. Immunogenicity of next-generation HPV vaccines in non-human primates: measles-vectored HPV vaccine versus Pichia pastoris recombinant protein vaccine. Vaccine. 2016;34:4724–31.
Zuniga A, Wang Z, Liniger M, Hangartner L, Caballero M, Pavlovic J, et al. Attenuated measles virus as a vaccine vector. Vaccine. 2007;25:2974–83.
Hu HM, Chen HW, Hsiao YJ, Wu SH, Chung HH, Hsieh CH, et al. The successful induction of T-cell and antibody responses by a recombinant measles virus-vectored tetravalent dengue vaccine provides partial protection against dengue-2 infection. Hum Vaccin Immunother. 2016;12:1678–89.
Xu P, Luthra P, Li Z, Fuentes S, D’Andrea JA, Wu J, et al. The V protein of mumps virus plays a critical role in pathogenesis. J Virol. 2012;86:1768–76.
Kubota M, Takeuchi K, Watanabe S, Ohno S, Matsuoka R, Kohda D, et al. Trisaccharide containing α2,3-linked sialic acid is a receptor for mumps virus. Proc Natl Acad Sci. 2016;113:11579–84.
Cui A, Zhu Z, Chen M, Zheng H, Liu L, Wang Y, et al. Epidemiologic and genetic characteristics of mumps viruses isolated in China from 1995 to 2010. Infect Genet Evol. 2014;21:384–90.
Ma C, Liu Y, Tang J, Jia H, Qin W, Su Y, et al. Assessment of mumps-containing vaccine effectiveness during an outbreak: importance to introduce the 2-dose schedule for China. Hum Vaccin Immunother. 2018;14:1392–7.
Fu C, Liang J, Wang M. Matched case-control study of effectiveness of live, attenuated S79 mumps virus vaccine against clinical mumps. Clin Vaccine Immunol. 2008;15:1425–8.
Liu Y, Hu Y, Deng X, Wang Z, Lu P, Ma F, et al. Seroepidemiology of mumps in the general population of Jiangsu province, China after introduction of a one-dose measles-mumps-rubella vaccine. Sci Rep. 2015;5:14660.
Billeter MA, Naim HY, Udem SA. Reverse genetics of measles virus and resulting multivalent recombinant vaccines: applications of recombinant measles viruses. Curr Top Microbiol Immunol. 2009;329:129–62.
Xu P, Chen Z, Phan S, Pickar A, He B. Immunogenicity of novel mumps vaccine candidates generated by genetic modification. J Virol. 2014;88:2600–10.
Liang Y, Ma J, Li C, Chen Y, Liu L, Liao Y, et al. Safety and immunogenicity of a live attenuated mumps vaccine: a phase I clinical trial. Hum Vaccin Immunother. 2014;10:1382–90.
Combredet C, Labrousse V, Mollet L, Lorin C, Delebecque F, Hurtrel B, et al. A molecularly cloned Schwarz strain of measles virus vaccine induces strong immune responses in macaques and transgenic mice. J Virol. 2003;77:11546–54.
Beaty SM, Park A, Won ST, Hong P, Lyons M, Vigant F, et al. Efficient and robust paramyxoviridae reverse genetics systems. mSphere 2017;2:e00376–16.
Ammayappan A, Russell SJ, Federspiel MJ. Recombinant mumps virus as a cancer therapeutic agent. Mol Ther Oncolytics. 2016;3:16019.
Wang Q, Vossen A, Ikeda Y, Devaux P. Measles vector as a multigene delivery platform facilitating iPSC reprogramming. Gene Ther. 2019;26:151–64.
Singh M, Billeter MA. A recombinant measles virus expressing biologically active human interleukin-12. J Gen Virol. 1999;101–6.
Funding
This work was supported partly by the Natural Science Foundation for Young Scholars of Zhejiang Province (LQ19H100005).
Author information
Authors and Affiliations
Contributions
Conceived and designed the experiments: ZZ and YW. Performed the experiments: DZ, MZ, YW, RL, XH, CZ, CQ, and DZ. Analyzed the data: DZ, YW, DZ, and ZZ. Wrote the manuscript: DZ, YW, MZ, ZZ, and all other co-authors edited the manuscript. All the authors approved the final manuscript as submitted and agree to be accountable for all aspects of the work.
Corresponding author
Ethics declarations
Ethical approval
This study was approved by Children's Hospital, Zhejiang University School of Medicine (No. 2013119).
Conflict of interest
The authors have no financial relationships relevant to this article to disclose.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Zhou, D., Zhu, MY., Wang, YL. et al. Attenuated MuV-S79 as vector stably expressing foreign gene. World J Pediatr 15, 511–515 (2019). https://doi.org/10.1007/s12519-019-00287-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12519-019-00287-7